大肠腺癌p53基因突变与p53基因蛋白表达的关系

Relationship between p53 gene mutation and p53 protein expression in colorectal adenocarcinomas

目的为了解大肠癌p53基因突变与p53基因蛋白表达之间的关系。方法用RT－PCR－SSCP方法检测100例新鲜大肠腺癌组织P53基因突变，以PAb1801单克隆抗体（单抗）免疫组织化学（LSAB法）染色检测相同腺癌组织p53基因蛋白表达。结果在这100例大肠腺癌中，p53基因突变阳性率为51％，PAb1801单抗免疫组织化学染色（免疫级化）阳性率为62％。在p53基因突变检测阴性的49例中，24例PAb1801阴性，25例PAb1801阳性；51例p53基因突变检测阳性中，14例PAb1801阴性，37例PAb1801阳性，Fisher＇sexacttestP＝0．02。结论大肠腺癌p53基因突变是参与和影响p53基因蛋白表达的一个主要因素。

Objective To study the relationship between p53 gene mutation and p53 protein expression in colorectal carcinomas. Metltods p53 gene mutation was detected by RT-PCR-SSCP and p53 protein was deteded by monoclonal antibody PAb1801 in the same tissue. Results In 100 patients with colorectal carcinomas, 51 (51 % ) were PO8itive for p53 gene mutation,and 62 (62% ) were PAb1801 positive shown by immunohistochemistry staining (LSAB method ). In 49 patients without p53 gene mutaion, 24 were negative and 25 positive for PAb1801. Among the 51 patients with p53 gene mUtation, 14 were negative and 37 positive for PAb1801 (Fisher's exact test,P=0. 02). Conclusion The mutation of p53 gene in colorectal carcinomas is one of the main factors which participates and affects p53 gene expression.