人及大鼠胶质细胞源性神经营养因子cDNA的克隆与表达

Cloning and expression of human and rat glial cell linederived neurotrophic factor cDNA

目的：克隆与表达人及大鼠胶质细胞源性神经营养因子（ＧＤＮＦ）ｃＤＮＡ。方法和结果：用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）扩增了人及大鼠ＧＤＮＦ成熟序列的ｃＤＮＡ片段，并将人及大鼠ＧＤＮＦｃＤＮＡ重组到表达质粒ｐＢＰＬ中，分别在大肠杆菌中获得了较高表达。结论：人及大鼠ＧＤＮＦｃＤＮＡ的克隆与表达获得成功，为研究ＧＤＮＦ在神经损伤修复中的作用奠定了基础。

Objective: To clone and express human and rat glial cell linederived neurotrophic factor (GDNF) cDNA. Methods and Results: The cDNA encoding human and rat GDNF was amplified by RTPCR. Then the human and rat GDNF cDNA was cloned into the expression vector pBPL. When transfected into E.coli, the recombinant plasmid pBPL G expressed a 16 000 protein, matching with its deduced molecular mass. Conclusion: Successful cloning and expression of human and rat GDNF lay foundations for studying the role of GDNF in the lesion and repairing of nervous system.