强啡肽A_(1～13)与大鼠脑出血后脑水肿关系的实验研究

The relationship between dynorphin A1～13 and brain edema after collagenaseinduced intracerebral hemorrhage in rats

目的：建立稳定的大鼠脑出血模型，研究强啡肽Ａ１～１３（ＤｙｎＡ１～１３）对大鼠胶原酶诱导的脑出血后脑水肿的影响，寻找治疗脑出血后脑水肿的特异性药物。方法：脑立体定位下，大鼠大脑尾核内微量注射０．４ＩＵ细菌胶原酶；一般（ＨＥ）与神经元特殊（尼氏）染色后观察脑出血后病理改变；干湿质量法测定脑组织含水量。结果：大鼠尾核注射胶原酶可以造成稳定的大鼠脑实质出血，２４ｈ可形成脑血肿，其大小与注射剂量有关，脑出血后脑组织含水量与注射胶原酶的剂量呈正相关，ＤｙｎＡ１～１３可以减轻胶原酶诱导的脑出血后脑水肿，并与剂量有关。结论：脑实质内注射胶原酶可制备大鼠脑出血模型，ＤｙｎＡ１～１３对脑出血后脑水肿可能有一定的治疗作用。

Objective: To establish a model of experimental intracerebral hemorrhage to study the relation between dynorphin A and brain edema after intracerebral hemorrhage in rats and to find a medicine which can decrease brain edema. Methods: 2 μl saline containing 0.4 IU bacterial collagenase was injected into the left caudate nucleus of adult rats, intracerebral hemorrhage was histologically studied and water content was expressed as the percentage change between wet and dry weight . Results: By 24 h there were hematomas, the size of which depended on the amount of collagenase injected, water content was significantly increased 4, 24, 48 and 72 h after injection at the needle puncture site and posterior brain sections. Dynorphin A1～13 significantly reduced water content of brain area after collagenaseinduced intracerebral hemorrhage in rats. Conclusion: Collagenaseinduced intracerebral hemorrhage is a reproducible animal model for the study of the brain edema. Dynorphin A significantly reduces hemorrhagic brain edema.