维生素E、普罗布考对溶血卵磷脂引起的脑微血管细胞损伤和增殖的保护作用

Protective effects of vitamin E and probucol on the injury and proliferation of cerebral microvascular cells caused by Lαlysophosphatidycholine

目的：研究维生素Ｅ（ＶＥ）、普罗布考（ＰＲＢ）对溶血卵磷脂（ＬＰＣ）引起的脑微血管内皮细胞（ＢＣＭＥＣ）损伤的保护作用和脑微血管平滑肌细胞（ＢＣＭＳＭＣ）增殖的抑制作用。方法：以乳酸脱氢酶（ＬＤＨ）释放及结晶紫染色法分别测定ＢＣＭＥＣ损伤和ＢＣＭＳＭＣ的增殖。结果：ＶＥ５～５０μｍｏｌ／Ｌ时显著抑制ＬＰＣ５０ｎｍｏｌ／Ｌ引起的ＢＣＭＥＣ释放ＬＤＨ，释放率下降至（３６．８±１．０）％～（１６．２±２．９）％；显著抑制ＬＰＣ１０ｎｍｏｌ／Ｌ引起的ＢＣＭＳＭＣ增殖，抑制率为（３７．６±７．０）％～（６１．３±０．６）％。ＰＲＢ５～５０μｍｏｌ／Ｌ时显著抑制ＬＰＣ５０ｎｍｏｌ／Ｌ引起的ＢＣＭＥＣ释放ＬＤＨ，释放率下降至（３４．２±３．３）％～（１９．１±２．４）％；显著抑制ＬＰＣ１０ｎｍｏｌ／Ｌ引起的ＢＣＭＳＭＣ增殖，抑制率为（４０．９±２．４）％～（４７．０±３．７）％。结论：ＶＥ和ＰＲＢ对ＬＰＣ引起的ＢＣＭＥＣ损伤有保护作用，对ＢＣＭＳＭＣ增殖有抑制作用。

Objective: To investigate the protective effects of vitamin E(VE) and probucol (PRB) on the injury and proliferation of cerebral microvascular cells. Methods: The injury of bovine cerebral microvascular endothelial cells (BCMEC) was determined by the lactic dehydrogenase (LDH) release and the proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC) with crystal violet staining. Results: VE of 5 to 50 μmol/L inhibited BCMEC from releasing LDH induced by LPC of 50 nmol/L, releasing rate declined to (36.8±1.0) % to (16.2±2.9)%, and inhibited BCMSMC proliferating induced by LPC of 10 nmol/L, with inhibitory rate of (37.6±7.0)% to (61.3±0.6)%. PRB of 5 to 50 μmol/L inhibited BCMEC from releasing LDH induced by LPC of 50 nmol/L, releasing rate declined to (34.2±3.3)% to (19.1±2.4)%, and inhibited BCMSMC from proliferating induced by LPC of 10 nmol/L, with inhibitory proliferation rate of (40.9±2.4) % to (47.0±3.7) %. Conclusion: VE and PRB have protective effects on the injury of BCMEC and the proliferation of BCMSMC induced by LPC.