摘要
目的:观察长征1号(CZ-1)多器官保存液对兔肾低温保存后的形态学变化。方法:将新西兰雄性兔肾脏分别用2~4℃CZ-1液、UW液及HC-A液50~100ml灌注,待肾脏变白后,切断所有血管,将肾脏取下放入装有CZ-1液、UW液和HC-A液保存罐中2~4℃保存,供取材作形态学观察。结果:72h以内CZ-1液保存组肾脏组织光镜和电镜下形态学无明显改变,而且CZ-1液保存组低温保存肾脏组织的变化以及细胞和细胞器的变性均缓于UW液与HC-A液保存组。结论:CZ-1液能低温保存肾脏在72h之内。
Objective:To observe morphological changes of cold-stored rabbit kidneys flushed with a new multi-organ preservation solution( CZ1) . Methods: Morphological investigations were performed using light microscopy and transmission electron microscopy in rabbit kidneys flushed with CZ1,UW and HCA solution at 0,24,48, 60, 64,72, 80 h of cold storage as compared with UW and HCA solution. Results: There was no significant changes within 72 h between CZ1 and UW solution. The morphological changes of rabbit kidneys flushed with CZ1 solution were superior to that with HCA solution. Conclusion: CZ1 solution could make rabbit kidneys coldstored for 72 h.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第1期57-59,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
多器官保存液
低温保存
肾移植
形态学
multi-organ preservation solution
kidney
cold preservation