自制多器官保存液对兔肾低温延时保存的形态学观察

The morphological observation on cold-stored rabbit kidneys flushed with a new multi-organ preservation solution

目的：观察长征１号（ＣＺ－１）多器官保存液对兔肾低温保存后的形态学变化。方法：将新西兰雄性兔肾脏分别用２～４℃ＣＺ－１液、ＵＷ液及ＨＣ－Ａ液５０～１００ｍｌ灌注，待肾脏变白后，切断所有血管，将肾脏取下放入装有ＣＺ－１液、ＵＷ液和ＨＣ－Ａ液保存罐中２～４℃保存，供取材作形态学观察。结果：７２ｈ以内ＣＺ－１液保存组肾脏组织光镜和电镜下形态学无明显改变，而且ＣＺ－１液保存组低温保存肾脏组织的变化以及细胞和细胞器的变性均缓于ＵＷ液与ＨＣ－Ａ液保存组。结论：ＣＺ－１液能低温保存肾脏在７２ｈ之内。

Objective:To observe morphological changes of cold-stored rabbit kidneys flushed with a new multi-organ preservation solution( CZ1) . Methods: Morphological investigations were performed using light microscopy and transmission electron microscopy in rabbit kidneys flushed with CZ1,UW and HCA solution at 0,24,48, 60, 64,72, 80 h of cold storage as compared with UW and HCA solution. Results: There was no significant changes within 72 h between CZ1 and UW solution. The morphological changes of rabbit kidneys flushed with CZ1 solution were superior to that with HCA solution. Conclusion: CZ1 solution could make rabbit kidneys coldstored for 72 h.