摘要
目的：观察离断肢体骨骼肌能量代谢的变化并探讨其临床意义。方法：采用３１Ｐ磁共振波谱技术对大鼠离断肢体在３６℃环境下的能量代谢变化进行测定。结果：随肢体缺血时间的延长，骨骼肌细胞内ｐＨ值、磷酸肌酸（ＰＣｒ）含量及ＡＴＰ含量降低，与此同时，细胞内无机磷（Ｐｉ）含量却迅速增加。ＰＣｒ／Ｐｉ比值在缺血后降低幅度则更加明显。结论：采用３１Ｐ磁共振波谱技术能够迅速准确地测定离断肢体骨骼肌组织的能量代谢动态变化，对于判断离断肢体的变性程度以决定是否予以再植具有明确的临床应用价值。
Objective: This study was designed to investigate energy metabolism in skeletal muscle of amputated limb and its clinical relevance. Methods: Energy metabolism of hind limbs in rats at 36℃ was defined with ^(31)P magnetic resonance spectroscopy. Results: During limb ischemia, intracellular pH, PCr and ATP levels decreased while intracellular Pi increased. As a result, a significant decrease in PCr/Pi ratio was also demonstrated. Conclusion: The dynamic changes in energy metabolism of skeletal muscles of amputated limbs may be rapidly and accurately monitored with ^(31_)P magnetic resonance spectroscopy so that the metabolic deterioration of muscle in the amputated limb and indication for replantation will be determined.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第1期63-65,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
截肢
创伤性
磁共振
骨骼肌
能量代谢
amputation, traumatic
muscles
ischemia
pH
phosphates
nuclear magnetic resonance
