摘要
目的:探讨IL-11对中子照射后肠上皮内Jak/STAT通路的影响,并观察其对Bax和Bcl-2表达的影响。方法:BALB/c小鼠和IEC-6细胞经4Gy中子照射和IL-11处理分别作为肠上皮损伤的体内外模型,采用HE染色、Western blot、EMSA、免疫组化和图像分析技术分别检测肠道病理变化、Jak1和STAT3活性及Bax和Bcl-2的表达。结果:(1)中子照射后,小鼠肠道损伤极为严重,且未见明显再生;IL-11治疗组隐窝上皮细胞及存活隐窝数量较多。(2)中子照射后,IEC-6细胞Jak1和STAT3活性均减弱,IL-11处理可增加二者活性。(3)中子照射后,小鼠肠道Bax表达增加,Bcl-2表达无明显改变;应用IL-11可降低Bax表达,增加Bcl-2表达。结论:在中子辐射时,IL-11可通过激活Jak/STAT通路,并下调Bax表达,上调Bcl-2表达,对肠上皮起到一定的保护作用。
AIM： To explore the effect of IL-11 on the activation of Jak/STAT pathway and the expressions of Bax and Bcl-2 in the intestinal epithelial cells exposed to neutron radiation. METHODS： The BALB/c mice and IEC-6, irradiated by 4 Gy neutron with or without IL-11 treatment, served as in vivo and in vitro model seperately. The changes of the intestines, activity of Jak1 and STAT3 and expressions of Bax and Bcl-2 were observed by HE staining, Western blot, EMSA, immunohistochemistry and image analysis. RESULTS： （1）Mice exposed to neutron radiation showed severe intestinal damages and no obvious regeneration was seen. IL-11-treated mice had a larger number of cryptal epithelial cells and crypts. （2）Neutron radiation decreased the activities of Jakl and STAT3, while IL-11 increased their activities. （3） Neutron radiation decreased the expression of Bax and didn＇t change the level of Bcl-2 in the murine intestine. IL-11 administration decreased the expression of Bax and increased that of Bcl-2. CONCLUSION： The mechanism of the intestinal protection of IL-11 in neutron irradiation might be that IL-11 stimulation triggered activation of Jak/STAT pathway, downregulated the expression of Bax and upregulated the expression of Bcl-2.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2009年第1期27-30,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30700197
30370438)
