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利用重组自交系群体对黄瓜侧枝相关性状进行QTL定位分析 被引量:6

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摘要 侧枝与黄瓜(Cucumis sativus)产量有密切关系.本实验利用侧枝长势较弱、萌发较早的华北类型品系S94和侧枝长势较强、萌发较晚的欧洲类型品系S06构建的224个F6:7家系进行黄瓜侧枝相关性状的研究.利用已构建的重组自交系群体遗传图谱,使用软件WinQTLCart2.5进行复合区间定位.在2006年秋和2007年春两季,共检测到4个侧枝相关性状(侧枝均长LBAL、侧枝总长LBTL、侧枝数目LBN和第一侧枝节位FLBN)的36个QTL,单个QTL的贡献率在3.1%(lbtl2.1,春季)~32.3%(lbn2.3,春季)之间.结果显示,4个不同性状的11个QTL(lbal1.1,lbtl1.1,lbn1.2,flbn1.2等)在两季中都聚集在第1连锁群e23m18d^ME23EM6c之间(7.4cM),并且在第2连锁群的S94A1~ME4SA4a之间(13.9cM)也检测到了4个不同性状的15个QTL(lbal2.1,lbtl2.1,lbn2.1和flbn2.1等).两季共有21个QTL贡献率超过10%,其中lbn2.3的贡献率(春季32.3%,LOD=18.4)为最大,lbtl1.3(秋季26.2%,LOD=17.4;春季26.9%,LOD=17.9)在两季的位置和贡献率都稳定.这些基因座为将来进行QTL精细定位提供了参考,同时利用其紧密连锁(<10cM)的特异标记(CMBR40,F,CS30,S94A1,CSWTA11B)可进行黄瓜侧枝性状的分子标记辅助育种.
出处 《中国科学(C辑)》 CSCD 北大核心 2008年第10期982-990,共9页 Science in China(Series C)
基金 上海市重点学科建设(批准号:B209)资助项目
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