摘要
目的:将医用臭氧注射到大鼠受损坐骨神经周围,观察对大鼠受损坐骨神经功能及病理形态改变的影响。方法:40只SD大鼠建立坐骨神经慢性松结扎损伤(CCI)模型后随机分成5组:Ⅰ模型对照组,Ⅱ臭氧20μg/ml治疗组,Ⅲ臭氧40μg/ml治疗组,Ⅳ臭氧60μg/ml治疗组,Ⅴ臭氧80μg/ml治疗组。分别在术前、术后第1、3、5、7、14、21、28天测定所有大鼠热刺激收缩反应期(TWL),于7、14、21、28天测定所有大鼠坐骨神经功能指数(SFI)及运动神经传导速度(MNCV),于28天处死动物进行形态学观察。结果:造模后第3天开始,大鼠出现热痛敏现象,对照组大鼠TWL差值与术前比较,第3、5、7、14、21、28天均明显增大(P<0.01)。Ⅲ、Ⅳ、Ⅴ组于第14、21、28天时点TWL与对照组相比均明显减小(P<0.01),各组间同一时点SFI、MNCV及光镜下坐骨神经病理形态改变差异无统计学意义。结论:40～80μg/ml臭氧局部注射可能对CCI模型鼠的热痛敏具有一定的治疗作用,但受损坐骨神经功能及病理形态改变无明显改善。
Objective ： To investigate the effects of local injection of medical ozone （ 03 ） on injured sciatic nerves. Methods：To establish the rat model of chronic constriction injury（CCI） on sciatic nerves,40 Sprague-Dawley rats were randomly divided into five groups： Ⅰ , control group; Ⅱ , with O3 20μg/ml; Ⅲ with O3 40 μg/ml; Ⅳ, with O3 60 μg/ml; Ⅴ, with O3 80 μg/ml. Thermal withdrawal latency （TWL） of rats were determined by the radiant heat stimulation on preoperation and 1 st, 3rd, 5th, 7th, 14th, 21th, 28th day after operation. The sciatic nerve functional index （SFI）and electrophysiological assessment （MNCV） were measured on 7th, 14th,21 th,28th day. The patho-morph results after killing rats were observed. Results：The allodynia to radiant heat stimulation on the territory of ligated sciatic nerves was found from the 3rd day after operation. There was significant difference （P 〈 0.01 ） in TWL between before and after operation. TWL in Ⅲ, Ⅳ and Ⅴ group was significantly different compared with control group（P 〈0.01 ）. The SFI and MNCV were no statistical difference in among groups. Conclusion： Medical ozone by local injection was probably effective for neuropathic pain of rats and it may not bring significant adverse effects on sciatic nerve function and neuropathology.
出处
《中国疼痛医学杂志》
CAS
CSCD
北大核心
2008年第6期341-344,347,共5页
Chinese Journal of Pain Medicine
基金
贵州省科学技术基金资助项目(黔科合J字[2007]2103)
