毛蕊花苷和异毛蕊花苷对树突状细胞增殖的影响

Effects of verbascoside and isoverbascoside on proliferation of dendritic Cells

目的观察大粒车前子提取物苯乙醇苷类化合物毛蕊花苷(verbascoside)和异毛蕊花苷(isoverbascoside)对小鼠骨髓来源树突状细胞增殖的影响。方法采用噻唑盐(MTT)法,以细胞因子(rmGM-CSF+rmIL-4)作为阳性对照,观察不同浓度的毛蕊花苷和异毛蕊花苷对小鼠骨髓来源树突状细胞增殖的影响。结果毛蕊花苷和异毛蕊花苷均可促进小鼠树突状细胞的增殖,与阳性对照组比较,毛蕊花苷在1.60×10-7～1.60×10-4mol·L-1内,异毛蕊花苷在所有浓度范围内作用效果均非常显著(P<0.05)。毛蕊花苷和异毛蕊花苷在1.60×10-6和1.60×10-5mol·L-1的浓度与细胞因子联用时,可显著刺激小鼠树突状细胞的增殖(P<0.05)。结论毛蕊花苷和异毛蕊花苷不仅可以直接促进小鼠骨髓来源树突状细胞的增殖,而且与细胞因子有明显的协同作用。

OBJECTIVE To investigate the effects of verbascoside and isoverbascoside on proliferation of murine bone marrow-devried dendritic cells. METHODS The tetrazolium salt reduction （MTr） assay was used to investigate the effect of verbascoside or isoverbascoside on proliferation of murine bone marrow-devried dendritic cells, and the cytokine （rmGM-CSF ＋ rmlL-d） was used as positive control. RESULTS Both verbascoside and isoverbascoside stimulated the proliferation of DCs significantly. Verbascoside had promoting effect in the concentration range of 1.60 × 10^ -7 - 1.60 × 10^ -4 mol · L^ -1 （ P 〈 0. 05 ）, while isoverbascoside also showed promoting effect in all test concentrations （P 〈 0. 05）. Further more, verbascoside and isoverbascoside at the concentrations of 1.60 × 10^-6 and 1.60 10^-5 mol/L stimulated the proliferation of murine bone marrow-devried dendritic ceils significantly （P 〈 0. 05 ）, white being coordinated with cytokine （rmGM-CSF ＋ rmIL-4 ）. CONCLUSIONS Verbascoside and isoverbascoside could induce the proliferation of murine bone marrow-devried dendritic cells, and show synergistic effect with the coordination with cytokine.