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羟乙葛根素对大鼠局灶性脑缺血再灌注损伤后脑组织E-selectin及P-selectin表达的影响 被引量：3

Effect of Hydroxyethylpuerarin on Expression of E-selectin and P-selectin in Rats with Focal Cerebral Ischemia-Reperfusion Injury
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摘要 目的观察E-selectin及P-selectin在羟乙葛根素保护大鼠局灶性脑缺血再灌注损伤中的作用。方法线栓法制备大鼠局灶性脑缺血再灌注损伤模型,大鼠脑缺血2 h再灌注24 h后,采用Western blot及RT-PCR法观察大鼠脑组织E-selectin及P-selectin的蛋白及mRNA表达情况。结果羟乙葛根素可明显降低缺血区脑组织E-selectin及P-selectin的蛋白及mRNA表达。结论羟乙葛根素可能通过降低缺血区脑组织黏附分子表达发挥脑保护作用。 OBJECTIVE To observe the effects of hydroxyethylpuerarin on expression of E-selectin and P-selectin in rats with focal cerebral ischemia-reperfusion injury and explore its action mechanism. METHODS Rats were subjected to cerebral ischemiareperfusion injury by middle cerebral artery occlusion （MCA0）. After a 2 h ischemia and 24 h reperfusion, the protein and mRNA expression of E-selectin and P-selectin were evaluated by western blot and RT-PCR analysis. RESULTS Hydroxyethylpuerarin significantly reduced the expression of E-selectin and P-selectin in ischemic brain tissue induced by cerebral ischemia-reperfusion. CONCLUSION Hydroxyethylpuerarin may attenuate brain ischemia injury by down-regulating the expression of adhesion molecules in ischemic brain tissues.
作者 娄海燕 魏欣冰 刘慧青 王姿颖 张岫美
机构地区 山东大学医学院药理学研究所
出处 《中国药学杂志》 CAS CSCD 北大核心 2008年第23期1788-1791,共4页 Chinese Pharmaceutical Journal
关键词 羟乙葛根素 脑缺血再灌注损伤 E-SELECTIN P-SELECTIN hydroxyethylpuerarin cerebral ischemia-reperfusion injury E-selectin P-selectin
分类号 R965 [医药卫生—药理学]
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参考文献8

  • 1JEAN W C,SPELLMAN S R,NUSSBAUM E S,et al. Reperfusion injury after focal cerebral ischemia: the role of inflammation and the therapeutic horizon [ J ]. J Neurosurg, 1998,43 ( 6 ) : 1382- 1397.
  • 2OKADA Y, COPELAND B R, MORI E,et al. P-selectin and intercellular adhesion molecule-1 expression after focal brain ischemia and reperfusion[J]. Stroke, 1994,25 ( 1 ) : 202-211.
  • 3SUZUKI H,ABE K,TOJO S,et al. Expressions of P-selectin and HSP72-like immunoreactivities in rat brain after transient middle cerebral artery occlusion [ J ]. Brain Res, 1997,759 ( 2 ) : 321- 329.
  • 4PANTONI L, SARTI C, INZITARI D. Cytokines and cell adhesion molecules in cerebral ischemia: experimental bases and therapeutic perspectives [ J ]. Arterioscler Thromb Vasc Biol, 1998, 18:503-513.
  • 5WANGZY WEIXB CHENL etal.Neuroprotective effects of hydroxyethylpuerarin against focal cerebral ischemia-reperfusion in rats.中国生理学报,2007,50(5):1-6.
  • 6LOUHY ZHANGXM WEIXB etal.Anti- inflammatory effect of hydroxyethylpuerarin on focal brain ischemia/reperfusion injury in rats .中国生理学报,2004,47(4):203-207.
  • 7王姿颖,魏欣冰,孙茹,孙霞,张岫美,仲英,左春旭.羟乙葛根素对大鼠脑缺血再灌注损伤一氧化氮和一氧化氮合酶的影响[J].中国药学杂志,2006,41(2):112-114. 被引量：13
  • 8LONGA E Z,WEINSTEIN P R,CARLSON S,et al. Reversible middle cerebral artery occlusion without craniectomy in the rats [ J ]. Stroke, 1989,20:84-91.

二级参考文献8

  • 1SUNR ZHANGXM WEIXB etal.Protective effects of compound N—2035 on focal brain ischemia—reperfusion injury in rats Antiapoptosis effects[J].山东大学学报：医学版,2003,41(3):231-233.
  • 2LOU H Y,ZHANG X M,WEI X B,et al.Anti-inflammatory effect of hydroxyethylpuerarin on focal brain ischemia/reperfusion injury in rats[J].Chin J Physiol,2004,47(4):204-207.
  • 3LONGA E Z,WEINSTEIN P R,CARLSON S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
  • 4DAWSON V L,DAWSON T M,LANDON E D,et al.Nitric oxide mediates glutamate neurotoxicity in primary cortical cultures[J].Proc Natl Acad Sci USA,1991,88:6368-6371.
  • 5DAWSON V L,BRAHMBBATT H P,MONG J A,et al.Expression of inducible nitric oxide synthase causes delayed neurotoxicity in primary mixed neuronal-glial cortical cultures[J].Neuropharmacology,1994,33(11):1425-1431.
  • 6DAWSON D A.Nitric oxide and focal cerebral ischemia:multiplicity of actions and diverse outcome[J].Cerebrovasc Brain Metab Rev,1994,6(4):299-324.
  • 7SAMDANI A F,DAWSON T M,DAWSON V L.Nitric oxide synthase in models of focal iyhemia[J].Stroke,1997,28:1283-1288.
  • 8王姿颖,张岫美,魏欣冰,孙茹,光红梅,王立祥.异黄酮类化合物N-2035对大鼠局灶性脑缺血再灌注脑组织损伤的保护作用(抗氧化活性)[J].山东大学学报（医学版）,2003,41(1):36-38. 被引量：14

共引文献12

同被引文献34

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二级引证文献4

中国药学杂志
2008年 第23期

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