HPLC测定人血浆中山莨菪碱的浓度

Determination of Anisodamine in Human Plasma by HPLC

目的建立高效液相色谱法测定人血浆中山莨菪碱的血药浓度。方法色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm);流动相为乙腈-10 mmol·L-1磷酸二氢钾溶液(pH3.0,内含10 mmol·L-1高氯酸)(20∶80);柱温:室温;流速:1.0mL·min-1;检测波长:214 nm。结果标准曲线的线性范围为0.5～50 mg·L-1(r=0.999 7,n=7),低、中、高3种浓度的平均回收率为(87.2±2.2)%,(92.5±1.0)%,(89.3±2.1)%,日内RSD≤2.5%,日间RSD≤5.2%。山莨菪碱的最低检出量为0.1 mg·L-1。结论本方法简单、灵敏、重现性好,适用于山莨菪碱临床血药浓度测定及人体药动学研究。

OBJECTIVE To establish a HPLC method for the determination of anisodamine in plasma. METHODS The HPLC separation was carried out on a Diamonsil Cls（4. 6 mm ×250 mm, 5μ i.tm） column , mobile phase was a mixture of acetonitrile and 10 mmol · L^-1 potassium dihydrogen phosphate buffer （pH 3.0,including 10 mmol · L^-1 perchloric acid ） （20： 80）. The flow rate was 1.0 mL · min^ -1. The detection wavelength was 214 nm. RESULTS A good linearity of anisodanime in plasma was obtained over the range of 0. 5 to 50 mg · L^-1 （ r = 0. 999 7 , n = 7 ）. Within the range, the recoveries of three different concentrations were （ 87. 2 ± 2. 2）%, （92. 5± 1.0）% , （89. 3± 2. 1 ）% , and the RSDs of intra-day and inter-day validation were less than 2. 5% and 5.2%, respectively. The detection limit of anisodamine was 0. 1 mg · L^-1. CONCLUSION The method is reproducible, convenient and sensitive for plasma concentration monitoring and clinical pharmacokinetic study of anisodamine.