不同废水基质条件下微生物燃料电池中细菌群落解析

Analysis of microbial diversity in microbial fuel cells under different wastewater

搭建了1个小型连续运行的无介体微生物燃料电池(MFC),分别以2种不同的有机废水为进水基质,均成功地实现了连续产电,同时对废水中的有机物也具有很好的去除效果.以葡萄糖为基质的微生物燃料电池的输出电压为435mV,以厌氧出水为基质的燃料电池的输出电压为475mV,2种基质系统中,COD去除率均达到60%以上.采用构建16S rRNA基因文库、随机测序的方法,对不同基质阳极表面的微生物群落结构进行研究.结果表明,产电阳极表面的细菌种类会发生很大变化,但其中几类与产电相关细菌的相对含量的变化不大,主要是低G+C革兰氏阳性细菌,变型细菌β亚纲(β-proteobacteria)和变型细菌δ亚纲(δ-protecobacteria)的细菌.本试验中的高产电细菌可能属于地杆菌科(Geobacteraceae).

A small microbial fuel cell （MFC） was operated continuously with two different influent, in which the COD was removed efficiently and electricity was generated simultaneously. The exported voltages for MFC were about 435 mV for MFC fed by glucose and 475 mV fed by anaerobic effluence,while COD removal efficiencies were all above 60%. With 16S rRNA genomic library construction and random sequencing,the diversity of microbes on anodes surface were detected respectively. Eubacteria community structures on anode surfaces changed greatly by different influent,but several relative electrochemically active microbes such as low G＋C gram positive bacteria, β-proteobacteria and δ-protecobacteria did not change much. The possible high electrochemically active bacteria belong to Geobacter.