优势菌在活性炭表面固定中生物量的变化规律研究

Biomass variation of dominant bacteria on activated carbon during immobilization

采用5株在松花江水中筛选驯化的优势工程菌,研究其在生物活性炭滤池固定化过程中菌种活性、固定方式、空床停留时间(EBCT)、pH值、菌液浓度以及温度对活性炭表面生物量的影响和变化规律.结果表明,固定化生物量与优势菌的特性有一定相关性,即菌种活性较高、环境温度较低、菌液pH值为酸性条件下,炭表面生物量越高;EBCT为60min,菌液浓度为1011个/mL的优势菌易于附着在活性炭上.采用单株菌依次固定与混合菌固定后,生物量没有明显差别,但菌种之间对活性炭表面栖息空间有一定的竞争作用.

The amount of dominant bacteria attached on activated carbon during immobilization would directly influence the running period and operational performance of immobilization biological activated carbon filter（IBAC）for start-up. Five kinds of dominant bacteria which were isolated from Songhua River and the acclimated species for the project had investigated for immobilization process, which including effects of biological activity, immobilization methods, empty bed contact time（EBCT）,pH value, microbial concentration and temperature on the biomass during immobilization. The attached biomass was higher under the condition of high biological activity, and low temperature and acidity, the biomass had relevance with microbial characteristics. It was easy for the dominant bacteria to attach on the activated carbon with EBCT of 60min and microbial concentration of 10^11 cfu/mL. The difference of biomass between the single sequential and the mixture immobilization was not obvious and the bacteria had the competition with each other for the living place on the surface of activated carbon.