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血管紧张素Ⅱ对RIN-m细胞PDX-1 mRNA及蛋白表达的影响 被引量：3

Effects of angiotensin Ⅱ on PDX-1 exprssion and insulin secretion of RIN-m cells
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摘要 目的探讨血管紧张素Ⅱ(ATⅡ)对胰十二指肠同源盒1(PDX-1)表达的影响及其与胰岛素(Ins)分泌的关系。方法测定RIN-m细胞在基础、ATⅡ及氯沙坦作用下Ins分泌量、细胞内Ins含量及细胞内PDX-1 mRNA和蛋白的表达水平。结果在100nmol/LATⅡ作用下,葡萄糖刺激后Ins分泌量、细胞内Ins含量及PDX-1表达水平均明显降低(P均<0.05)。上述作用可被ATⅡ受体阻滞剂氯沙坦部分逆转。结论抑制PDX-1表达水平可能是ATⅡ影响胰岛β细胞Ins合成及分泌的机制之一。 Objective To study the influence of angiotensin Ⅱ on the expression of PDX-1 and insulin secretion. Methods Basic and glucose-induced insulin secretion in supernate and in RIN-m cells were determined by RIA. The PDX-1 mRNA and protein were detected by in-situ hybridization and immunocytochemistry. Results （1） Pretreatment of the RIN-m cells with angiotensin Ⅱ significantly decreased the levels of glucose-stimulated insulin release, intracellular insulin content and PDX-1 expression. （2） Losartan partly improved PDX-1 expression and insulin secretion. Conclusions The angiotensin Ⅱ-induced impairment of PDX-1 mRNA and protein expressions may be one of the possible mechanisms for Ang Ⅱ to affect the insulin synthesis and secretion.
作者 刘敏 蔡德鸿 张桦 袁小澎 孙嘉 李明 张振 陈宏
机构地区 南方医科大学珠江医院内分泌科 移植免疫研究所
出处 《中国糖尿病杂志》 CAS CSCD 北大核心 2008年第12期749-751,共3页 Chinese Journal of Diabetes
基金 国家自然科学基金资助项目(编号30470829)
关键词 血管紧张素Ⅱ RIN—m细胞 胰岛素分泌 胰十二指肠同源盒1 Angiotensin Ⅱ RIN-m cells Insulin secretion Pancreatic and duodenal homeobox-1
分类号 R587.1 [医药卫生—内分泌] R544.1 [医药卫生—心血管疾病]
  • 相关文献

参考文献7

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二级参考文献12

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共引文献10

同被引文献30

  • 1孙嘉,蔡德鸿,张桦,卫国红,曾龙驿,翁建平,陈规划.血管紧张素Ⅱ与人离体胰岛功能研究[J].中华内分泌代谢杂志,2005,21(6):569-572. 被引量：11
  • 2邢军,许评,周毅,宋一民,李爱东,宋纯,宋春芳.氯沙坦对体外培养的犬胰岛细胞凋亡及凋亡相关基因表达的影响[J].中华器官移植杂志,2006,27(4):241-244. 被引量：2
  • 3邵加庆,岩下乃夕,杜宏,王燕燕,赵明,王坚,绵田裕孝,河盛隆造.血管紧张素Ⅱ受体阻断剂治疗对db/db糖尿病小鼠胰岛的作用[J].中华内科杂志,2007,46(4):306-310. 被引量：5
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引证文献3

二级引证文献1

中国糖尿病杂志
2008年 第12期

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