共轭亚油酸诱导人结肠癌细胞Caco-2凋亡的作用

Apoptosis in Human Colon Adenocarcinoma Cell Line Caco-2 Induced by the Mixture of Conjugated Linoleic Acids

共轭亚油酸(conjugated linoleic acid,CLA)有着多种异构体以及多种生理活性,如抗癌、减肥、抗动脉粥样硬化等。本研究主要对c9,t11-CLA和t9,t11-CLA两种异构体的混合物诱导人结肠癌细胞Caco-2凋亡的作用及可能机制进行了探讨。采用MTT方法、透射电镜观察、流式细胞术检测和RT-PCR方法,研究了CLA混合物抑制Caco-2细胞增殖,诱导Caco-2细胞凋亡的作用及可能机制。实验结果证明,c9,t11-CLA和t9,t11-CLA混合物能抑制Caco-2细胞的增殖,并可诱导Caco-2的凋亡。随着所用的CLA混合物浓度的增加以及作用时间的延长,细胞凋亡率增加。通过RT-PCR分析,Caco-2细胞中的caspase 3的mRNA的表达随着CLA混合物浓度的增加而上调。这两种CLA的混合物可抑制Caco-2细胞的增殖,诱导Caco-2的凋亡,而caspase 3的表达上调可能与细胞凋亡密切相关。

Conjugated linoleic acids（CLA） have many physiological activities. In the recent years, much attention has been paid to the anticancer effects of CLA. The present study was to investigate the effect of the mixture of c9 ,t11-CLA and t9, t11-CLA on the apoptosis of Caco-2 cell line. MTT assay, transmission electronmicroscopy, flow cytometry and RT-PCR were employed to determine the ability of the mixture CLA to induce apoptosis of Caco-2 cell line. The results showed that the mixture of the two CLA isomers inhibited the Caco-2 cell proliferation and induced cell apoptosis. The activity was closely related to the treatment time and concentration of the mixture. The results of transmission electronmicrograph and flow cytometery methods showed that the mixture of CLA induce dose-and time-apoptosis. The change of caspase 3 mRNA under addition of CLA was investigated by RT-PCR,and the results suggested that the mixture CLA induce caspase-apoptosis of Caco-2.