摘要
采用热水浸提,乙醇沉淀的方法得到大马勃粗多糖,经Sevag法脱蛋白,DEAE-sepharose fast flow离子交换层析及sephacryl S-300HR凝胶过滤法得大马勃多糖(CGPⅠ-1)。气相色谱研究表明,其单糖组成为:Gal,Glc,Man等四种单糖,其中Gal,Glc,Man,摩尔比为3.92∶11.28∶1.22。经部分酸水解,红外光谱及核磁共振光谱,高碘酸氧化,Smith降解等分析,CGPⅠ-1的主链由Man和Glc构成,存在β型和α型两种糖苷键构型,支链或主链的末端残基由β-Gal(1→4),β-Glc(1→6),α-Glc(1→4)构成,其分子中存在酰胺结构。原子力显微镜观察发现CGPⅠ-1呈分支的线性分子,在水溶液中容易互相缠绕形成强大的网络结构。
Calvatia geigantea polysaccharide CGP was extracted by hot water,precipitated by ethanol, and deproteinized by Sevag method. The CGP I -1, a main constituent of CGP, was obtained by DEAE-sepharose and Sephacryl S-300HR gel-chromatography. The acid hydrolyzed product and GC-MS analysis of the CGP I -1 showed that CGP I -1 is composed of Gal, Glc, Man and so on. Gal, Glc and Man in a molar ratio of 3.9: I 1.28: 1.22. IR analysis, partial hydrolysis with acid,periodate oxidation, Smith degradation, and ^1H NMR, ^13C NMR analysis revealed that the main chain of CGP I -1 is mainly made up of Man and Glc. The side chain or terminal residues is composed of β-Gal(1→4) ,β-Glc( 1→6) ,α-Glc( 1→4). There was amide group in CGP I -1. The molecular structure of the CGP I -1 at different concentrations was observed with atomic force microscopy(AFM). The CGP I -1 molecule had a linear backbone with branches. The net work structure of CGP I -1 was formed through entanglement between the molecules of the polysaccharide.
出处
《天然产物研究与开发》
CAS
CSCD
2008年第6期1027-1030,共4页
Natural Product Research and Development
关键词
大马勃
多糖
分离纯化
结构分析
Calvatia geigantea
polysaccharide
separation and purification
structure analysis