高效液相色谱建立掌叶大黄指纹图谱

Fingerprint Development for Rheum palmatum by HPLC

采用高效液相色谱法建立不同来源掌叶大黄的指纹图谱,为其质量控制提供依据。本实验方法采用Nuc leodur C18(4.6 mm×250 mm,5μm)色谱柱,流动相以甲醇:0.5%磷酸水溶液(1∶9,V/V)作为A相,乙腈作为B相进行梯度洗脱(流速0.8 mL/m in,检测波长280 nm)。通过聚类分析和相似度分析处理,14个不同来源的掌叶大黄样品被初步分为两大类:正品掌叶大黄和非正品。实验方法简便、可靠,可成为掌叶大黄质量评价的有效手段。

Fingerprint of Rheum palmatum L. collected from different producing areas was developed by HPLC for its quality control. The separation was carried out on a Nucleodur C18 （4.6 mm × 250 mm,5 μm） column with the mixture of 0.5% phosphate acid-methanol and aeetonitrile as mobile phase in a gradient elution model ,and the detection wavelength was at 280 nm. By using cluster analysis and similarity calculation, the 14 batches of rhubarb samples were classified as two types：the ordinary and the inferior. This method is simple, reliable, and providing a possibility for effective quality control of rhubarb.