白花蛇舌草豆甾醇对肝癌细胞的体内外抑制作用及对其增殖周期、凋亡的影响

Inhibitive effect of Stigmasterol from Hedyotis diffusa willd,on hepatoma cells in vitro & vivo and its influence on transplanted H22 tumor cell's multiplication cycle,apoptosis

目的:研究白花蛇舌草豆甾醇(stigmasterol from Hedyotis diffusa willd.,SHD)对人肝癌细胞SMMC-7721、BEL-7402的体外抑制作用,对肝癌H22的体内抑制作用及对其增殖周期、凋亡的影响。方法:MTT法评价SHD对人肝癌细胞SMMC-7721、BEL-7402的抑制率变化规律。昆明雄性小鼠60只,随机取10只为正常对照组,余接种H22瘤株,随机分为模型对照组、5-FU阳性对照组(30mg/kg)和高中低剂量SHD给药组(剂量分别为15、30、60mg/kg),腹腔给药10 d后,比较各组瘤重抑制率、H22细胞周期分布、凋亡率。结果:SHD对SMMC-7721、BEL-7402细胞具有体外抑制作用;SHD显著抑制H22肿瘤,增加G0-G1期细胞比例,降低G2/M期细胞比例,促进肿瘤细胞凋亡。结论:SHD在体外、体内均具有抑制肝癌细胞的作用,此作用与阻滞肿瘤细胞增殖周期,促进肿瘤细胞凋亡有关。

Objective：This study was designed to investigate inhibitive effect of Stigmasterol from Hedyotis diffusa willd.（SHD） on human hepatoma cell line SMMC-7721,BEL-7402 in vitro,its antitumor effect on transplanted H22 tumor cells in vivo and its influ- ence on the tumor cell＇s multiplication cycle,apoptosis.Methods：The cultured SMMC-7721 and BEL-7402 cells were treated with different concentrations of SHD.The proliferation of the cells was measured by MTT assay.The growth of transplanted H22 tumor in mice, the changes of its multiplication cycle and apoptosis were investigated.Results：The proliferation of SMMC-7721,BEL-7402 cells in vitro was inhibited by SHD.In H22 mice,transplanted tumor was suppressed,the proportion of G0-G1 cells was increased,G2/M cells were reduced,the tumor cell＇s apoptosis was induced.Conclusion：Hepatoma cells can he inhibited by SHD both in vivo and in vitro.The effects are related with blocking tumor cell multiplication cycle,inducing its apoptosis.