不同低氧运动模式对大鼠免疫功能的影响

Immunity of Rats in Different Hypoxic Training

目的:探讨不同低氧运动模式对大鼠免疫功能的影响。方法:30只SD大鼠随机分为常氧运动组(对照组)、高住高练组和高住低练组,对照组大鼠每天在常氧环境下生活23 h,训练1 h;高住高练组每天在低氧环境生活23 h,训练1 h;高住低练组每天在低氧环境生活12 h,常氧环境下生活11 h,训练1 h。全血分析仪分析全血指标,流式细胞仪检测CD4^+和CD8^+占有的百分比。结果:与高住低练组及对照组比较,高住高练组大鼠血液红细胞计数(RBC)和血红蛋白水平(Hb)升高,CD4^+T细胞百分数降低,CD8^+T细胞百分数升高,CD4^+/CD8^+比值下降,差异均具有统计学意义(P<0.05)。对照组大鼠、高住高练组和高住低练组ELISPOT斑点数依次为(230.0±10.6)/2×10~5个脾细胞、(56.5±6.8)/2×10~5个脾细胞和(89.3±9.8)/2×10~5个脾细胞,差异有统计学意义(P<0.05)。结论:低氧运动可以提高机体氧运输能力,但是可能会损害机体免疫功能。

Objective： To study different hypoxic environment effect on the immunological function in rats. Methods： The 30 rats were randomly divided into normal oxygen training group （control group）, living high-training high group （HiHo）, and living high-training low group （HiLo）. Control group lived under normal oxygen environment for 23 h a day, trained 1 h; HiHo group lived under hypoxic environment for 23 h a day, trained 1 h. HiLo group lived under hypoxic environment for 12 h, then lived under normal oxygen environment for 11 h a day, trained 1 h. Blood samples were assayed with auto cell analyzer, and the ration of CD4^＋ and CD8^＋ were detected by flow cytometry. Results： Compared with other two groups, the levels of RBC and Hb were heightened in the HiHo group, and the percent of CD4^＋ was decreased, the percent of CD8^＋ increased, CD4^＋/CD8^＋ reduced, all with significant differences（P〈0. 05）. The ELISPOT number of control group, HiHo group and HiLo group were respectively （230.0± 10.6）/2× 10^5, （56.5± 6.8）/2 × 10^5 and （89.3 ± 9.8）/2 × 10^5 splenic cell, with significant differences （P〈0.05）. Conclusions： Hypoxic training can improve the ability to transmit oxygen, which however may weaken the immunity of the rats.