抗SEA鸡卵黄抗体用于血吸虫循环抗原检测的初步研究

Detection of Circulating Schistosome Antigen with Anti-Sea Chicken Immunoglobulin Y

目的:探讨抗可溶性虫卵抗原(SEA)鸡卵黄抗体(Immunoglobulin Y,IgY)用于血吸虫循环抗原检测的可行性。方法:以纯化的鸡抗SEA卵黄抗体为捕捉抗体,以酶标抗SEA单克隆抗体NP28-5B进行双抗体夹心酶联免疫吸附试验法(S-ELISA)检测急、慢性血吸虫病病人和健康人血清,并与常规检测抗体的酶联免疫吸附试验(SEA-ELISA)法做比较。结果:S-ELISA法测得SEA浓度(y)与A450值(x)呈明显的正相关(r=0.9481,y=459.22x-108.14)。S-ELISA法检测急性血吸虫病人循环抗原的阳性率为100%,慢性血吸虫病人循环抗原的阳性率为84.40%,健康人的特异性为96%。两种方法对血吸虫病的检出率差异无统计学意义(P>0.05)。结论:S-ELISA可用于血吸虫病的免疫诊断。

Objective： To analyze the feasibility on detection of circulating schistosome antigen with anti-SEA chicken immunoglobulin Y （IgY）. Methods： Chicken anti-SEA IgY was used as the capture-antibody and an anti-SEA mouse mAb conjugated with HRP as the detection antibody, a sandwich ELISA technique was established to detect the circulating schistosome antigen in serum samples of patients with acute, chronic schistosomiasis and healthy people. The results were compared with SEA-ELISA. Results： There was an obviously positive correlation between SEA concentration and A450 value. The regression equation was deduced （r=0.9481,y=459. 22x-108.14）. The positive rates of S-ELISA for testing acute, chronic schistosomiasis patients were 100% and 84%, respectively. Its specificity for healthy people was 96%. No significant difference was observed between the two ELISA methods. Conclusion： S-ELISA could be used in immunodiagnosis of schistosomiasis.