产生鬼臼毒素菌株的诱变选育

Mutating of Podophyllotoxin High-producing Strain

目的:提高原始菌株鬼臼毒素的产量,以期组织工业化生产,从而扩大鬼臼类化合物的资源。方法:以交链孢霉Ty为研究对象,进行紫外线和He-Ne激光复合诱变,再对所获得高产菌株进行发酵条件的优化。结果:筛选到一株鬼臼毒素高产菌株Ty4-16-13,产量达4.213μg/L,比原始菌株提高96%。结论:诱变后的菌株经7次传代,产量较稳定,可用于工业化生产。

Objective： To enhance the podophyllotoxin output of the Primitive strain for industrialization production to expand the podophyllotoxin resources. Methods： Alternaria Nees ex wall Ty was treated by UV and He-Ne laser irradiation. Then we optimized fer- mentation condition of the mutant high production strain. Results： A mutant strain Ty4-16-13, with a podophyllotoxin output up to 4.213 μg/L, 96 percent higher than the Primitive strain, was screened out. Conclusion： The podophyllotoxin output of the mutant strain was very steady after cultured by seven times, so as to be used in the industrialization production.