HPLC法测定吴茱萸中吴茱萸碱和吴茱萸次碱的含量

Determination of Evodiamine and Rutaecarpine in Fructus Evodiae by HPLC

目的:建立比较简单可靠的高效液相色谱(HPLC)方法以测定吴茱萸药材中吴茱萸碱和吴茱萸次碱的含量。方法:采用乙酸乙酯超声提取方法制备样品。以乙腈-水-乙酸(51:48:0.1)为流动相,在波长225 nm处检测。结果:吴茱萸碱和吴茱萸次碱得到最优化的分离效果,其平均回收率分别为98.5%和96.0%,实际样品的检测结果令人满意。结论:本法简单、快速、准确、可靠,适用于吴茱萸药材的质量控制,

Objective： A simple and speedy high-performance liquid chromatography （HPLC） method with UV detection was established to determine the cvodiamine and rutaecarpine in Fructus Evodiae. Method： The samples were injected into the sampling valve and dealt with ultrasonic without tedious preconditioning. Chromatographic conditions included mobile phase acenitrile-H2O-acetate acid （51 ： 48 ： 0.1）, detecting wavelength at 225 nm. Results： Evodiamine and rutaecarpine was separated successfully. The mean recoveries of evodiamine and rutaecarpine were 95.8% and 96.0%, respectively. The results from real samples were satisfactory. Conclusion： This method is relatively simple, speedy and accurate, and is suitable for the routine analysis and quality control of Fructus Evodiae.