德氏乳杆菌亚油酸异构酶提取条件的研究

Research on extraction conditions of linoleic acid isomerase from Lactobacillus.delbrueckii subsp

对德氏乳杆菌中亚油酸异构酶的提取条件进行了研究,并对其粗酶液的性质进行了检测,为下一步的分离纯化工作提供依据。通过实验,确定了超声波破碎产亚油酸异构酶菌体的条件:间歇破碎,每次破碎时间20s,间歇5s,破碎20次。当缓冲液pH值为6.0,菌体质量浓度为0.50g/mL时,以粗酶液的酶活力为指标,确定反应的最佳条件:反应温度为40℃;反应时间为150min;粗酶液与底物比例为1:2。

Extraction conditions of Linoleic Acid Isomerase and properties of crude enzyme was studied in this paper. The optimum disruption condition for Lactobadllus.delbrueckii subsp was： intermittent ultrasonic treatment 20 times and time for each treatment was 20 s, with a 5 s interval. pH of the disruption buffer 6.0, concentration of cell extracted 0.50 g/mL Through determination activity of crude enzyme confirm that reaction temperature 40℃, reaction time 150min, proportion of enzyme and substrate 1：2.