邻苯二甲酸丁基苄酯对小鼠肝脏DNA-蛋白质交联水平的影响

Effect of Butylbenzyl Phthalate(BBP) on Levels of DNA-protein Crosslinks in Hepatic Cells of Mice

目的研究邻苯二甲酸丁基苄酯(butylbenzyl phthalate,BBP)对小鼠肝脏细胞DNA-蛋白质交联(DNA-protein crosslinks,DPC)水平的影响。方法将25只SPF级昆明纯系雄性小鼠随机分为5组,每组5只,分别为125、250、500、1 000mg/kgBBP染毒组和对照组。BBP腹腔注射染毒,每天1次,两周后,分离肝脏,检测小鼠肝细胞DPC系数。结果随着BBP染毒剂量的增高肝细胞DPC系数随之升高。与对照组比较,当BBP染毒剂量为500和1 000mg/kg时,染毒组小鼠肝细胞DPC含量升高,差异有统计学意义(P<0.01)。结论低剂量(125 mg/kg)的BBP不引起小鼠肝细胞的DPC效应,而较高剂量(500和1000 mg/kg)的BBP则能引起显著的DPC效应,造成严重的DNA损伤。

Objective To understand the effect of butylbenzyl phthalate （BBP） on the levels of DNA-protein crosslinks（DPC） in hepatic cells of mice. Methods Twenty-five male Kunming mice were randomly assigned into five groups （n=5）. BBP was administered by intraperitoneal injection at doses of 0 （control group）, 125, 250, 500, 1 000 mg/kg respectively for 14 consecutive days. Then the levels of DNA-protein crosslinks were detected. Results DPC coefficient increased with concentration of BBP increasing. Treatments of 500 and 1 000 mg/kg BBP significantly increased the levels of DPC compared with the control group. Conclusion BBP can not cause DPC at low levels （125 mg/kg）, but it can cause DPC significantly at high levels （500 and 1 000 mg/kg）, which may cause serious DNA damages.