模拟失重对培养心肌细胞形态和结构的影响

A SIMULATION STUDY ABOUT EFFECTS OF MICROGRAVITY ON CELLULAR MORPHOLOGY AND STRUCTURE OF RAT'S CARDIAC MUSCLE

本实验是利用回转器模拟失重对离体培养大鼠乳鼠的心肌细胞形态和结构的影响．在光学显微镜和荧光显微镜下观察发现，细胞的形态由细长梭形变成椭圆形甚至为圆形，并且通过荧光标记后的细胞骨架的排列由纵形变成辐射状．同时在对细胞进行测量发现，细胞体积缩小近40％，细胞长短径比例减少近70％．上述结果提示模拟失重对培养心肌细胞的形态和结构有显著影响．

A laboratory simulation is made to show the effects of microgravity on the cellular shape and structure of rat's cardiac muscle. The cell's cultural system is put into a simulated microgravity environment. It is found that cellular morphology changes from slim to round in optical microscope and cytoskeloton changes from columnar morphology to radial morphology through marked Actin in fluorescence microscopy. Cellular area of cardiac muscles becomes significandy decreased by 40% and cellular long-short axes ratio decreased by 70% through micromeasurement.