鸡胸软骨硫酸软骨素的制备及结构分析

Preparation and Structure Analysis of Chondroitin Sulfate from Chicken Sternal Cartilage

首先分析了鸡胸软骨中主要营养成分,再用酶法提取生物活性多糖硫酸软骨素,并用三氯乙酸除蛋白、乙醇沉淀和柱色谱进行分离纯化得到高纯度产品。反相高效液相色谱和原子吸收光谱分析表明鸡胸软骨富含呈味氨基酸和钙、镁和锌等矿物元素。高效凝胶过滤色谱和琼脂糖凝胶电泳证明该纯化后的硫酸软骨素产品均一,纯度达到99%,其分子量为21007D。其红外光谱呈现4-硫酸软骨素的典型特征,没有发现6-硫酸软骨素存在。气相色谱分析发现其中中性单糖主要为葡萄糖、木糖和半乳糖,与猪喉软骨存在明显差异。

In this study, the nutrients in chicken sternal cartilage were analyzed, and chicken sternal cartilage was hydrolyzed with papain. After protein removal with trichloroacetic acid, chondroitin sulfate was precipitated from chicken sternal cartilage hydrolysate, and then was purified with 732 anion exchange chromatography and Sephacryl-300 HR gel filtration chromatography in turn. Reversed-phase high-performance liquid chromatography （RP-HPLC） and atomic absorption spectrum analyses indicated that chicken sternal cartilage contains many flavour amino acids and beneficial mineral elements such as calcium, magnesium and zinc. The data of high performance gel filtration chromatography （HPGFC）and agarose gel electrophoresis （AGE） exhibited that the obtained chondroitin sulfate is of high purity （99%）, and its molecular weight is 21007 D, which is smaller than that from pig laryngeal cartilage. Infrared spectrum analysis showed that it is chondroitin-4-sulfate but not chondroitin-6-sulfate. Gas chromatography analysis proved that its main neutral monosaccharide compositions are glucose, xylose and galactose, which are obviously different from those of chondroitin sulfate from pig laryngeal cartilage.