正交设计优选白芨多糖包合丹皮酚最佳工艺以及包合物的鉴定

Study on Optimization of Inclusion Process of Paeonol with Bletilla striata(Thunb.) Reichb. f. Polysaccharides(BSPS) by Orthogonal Design and Identification of Inclusion Complex

本实验采用L9(34)正交试验优选白芨多糖包合丹皮酚的最佳工艺,并通过热差示分析仪、红外图谱和核磁共振氢谱对包合物进行鉴定。结果显示:以丹皮酚和白芨多糖的物料比、反应时间和反应温度为考察指标,得到优化工艺为:物料比1:6、反应时间4h、温度30℃,包封率可达29.38%,收得率74.29%。差示热分析仪、红外图谱和核磁共振氢谱的鉴定结果表明:丹皮酚的白芨多糖包合物已经形成,同时对单独使用白芨多糖包合丹皮酚的机理作出探讨,以期为丹皮酚与白芨多糖生物学活性的叠加提供理论依据。

Orthogonal test design L9（3^4） was used to optimize the preparation process of inclusion compound ofpaeonol-BSPS in this study, and differential thermal analysis meter （DTA）, IR-spectrtma and nuclear magnetic resonance （NMR）. were used to identify the inclusion. Results showed that the optimal preparation process of inclusion are as follows： ratio of paeonol-BSPS 1：6, temperature 30 ℃, water bath time 4 h, and by this process the inclusion rate and yield are 29.38% and 74.29%, respectively. The results of DTA, iR-spectrum and NMR showed that the inclusion has formed. The mechanism of inclusion of paeonol with BSPS was discussed.