摘要
本实验采用CO2超临界流体萃取法(CO2-SFE)和乙醇回流法提取大高良姜的有效成分1'-乙酰氧基胡椒酚乙酸酯(ACA),通过硅胶柱纯化前后TLC图谱、HPLC图谱、有效成分ACA的含量及萃取物的物理指标为指标评价两种方法。结果表明:CO2-SFE提取物所含杂质较少,萃取的挥发油中ACA的含量达72.11%。CO2-SFE法得到ACA的净含量是乙醇回流法的5.6倍。用CO2-SFE提取大高良姜中的ACA明显优于乙醇回流法。
CO2- SFE and ethanol refluxing methods were repectively used to extract 1＇-acetoxychavicol acetate （ACA） from Alpinia galanga （L.） Swartz. The qualities of ACA extracts with the two methads were evaluated by TLC and HPLC chromatogram, effective composition content of ACA and physical index respectively. The results showed COa-SFE extract is purer, and the content of ACA is 72.11% in the volatile oil of the extract, and the net content of ACA is 5.6 times of that in ethanol refluxing extarct. In conclusion, CO2-SFE is obviously better than ethanol refluxing method for extracting effective componen ACA from Alpinia galanga （L.） Swartz.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第12期292-295,共4页
Food Science
基金
教育部“长江学者和创新团队发展计划”项目(IRT0540)
关键词
CO2超临界流体萃取法
乙醇回流法
ACA
CO2-supercritical fluid extraction （SFE）
ethanol refluxing method
ACA
