摘要
实验研究了不同色谱条件下食品中常见的反油酸、反异油酸及反亚油酸的分离效果,确定的色谱条件为:HP-88毛细管色谱柱(60m×0.25mm,0.2μm);柱温:恒温180℃;检测器:250℃;进样器:250℃;载气:高纯氮,压力:100kPa,流速:16ml/min;氢气压力:100kPa,流速:28ml/min;空气压力:160kPa,流速:200ml/min;分流比:30:1;尾吹流速:18ml/min,在此操作条件下30min内能较好的分离顺/反式油酸甲酯(C18:1,c9/C18:1,t9)及其同分异构体反异油酸甲酯(C18:1,t11)和顺/反式亚油酸甲酯(C18:2,c9c12/C18:2,t9t12)。在0.05～0.4mg/ml浓度范围内线性良好(r>0.998);三种反式脂肪酸的检测限分别为:6.84×10-15、8.28×10-15、6.57×10-15g/s。从甲酯化处理至气相色谱检测,方法的回收率在85.29%～86.41%范围间,相对标准偏差在1.47%～8.50%之间。
In the present study, the separatation effects of different elaidic acid, trans-vaccenic acid, and linolelaidic acid mainly contained in food under different gas chromatography（GC） conditions. It was found experimentally that the optimal GC condition are as follows： HP-88 capillary colunm （60m × 0.25mm, 0.2 μm）, column temperature 180 ℃; sampler temperature： 250 ℃, detector temperature： 250 ℃, pure N2 as carrier gas, pure Nz pressure 100kPa, plue N2 flow rate： 16 ml/min, hydrogen pressure 100 kPa, hydrogen flow rate 28ml/min, air pressure 160kPa, air flow rate： 200 ml/min, split injection mode with the split ratio of 30：1 and tail-blows rate 18 ml/min. Elaidic acid （C18：1, c9）, linolelaidic acid （C18：2, t9t12）and trans-vaccenlc acid （C18：1, t11） can be separated completely within 30 rain under these operation conditions. And the linear scopes are between 0.05mg/ml and 0.4mg/ml （r〉0.998）. The detection limits of the three trans fatty acid are 6.84 ×10^-15 g/s, 8.28 ×10^-15 g/s, and 6.57 ×10^-15 g/s, respectively. The recovery rate of the method from esterification to GC detection is between 85.29% and 86.41% with the relative standard deviation is between 1.47%and 8.50%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第12期511-515,共5页
Food Science
基金
上海市科委世博专项项目(SA150158)
