高效液相色谱法测定能量饮料中多种水溶性维生素

Eestablishment of HPLC Method for Determination of Seven Water-soluble Vitamins in Energy Beverage

研究利用反相液相色谱法测定能量饮料中七种水溶性维生素(VC、VPP、VB1、VB2、VB5、VB6、叶酸)的方法。样品利用Supelco C18固相萃取柱(500mg/6ml)萃取后,直接注入高效液相色谱柱Phenomenex C18(250mm×4.6mm,5μm),采用等度洗脱,流动相为0.1mol/L KH2PO4(pH7.0)-甲醇(90:10,V/V),流速为0.5ml/min。根据各个化合物的保留时间以及UV谱图与标准谱图的对比可以确认各化合物。该方法的检测限为0.05～0.2mg/L,平均回收率为94.71%～97.03%。

A reversed-phase high-performance liquid chromatography（HPLC） method was developed for determination of water-soluble vitamins （ascorbic acid, niacin, panthothenic acid （vitamin B5）, pyridoxine （vitamin B6）, thiamine （vitamin B1）, folic acid, and riboflavin （vitamin B2） in an energy beverage. Analytes were separated after solid phase extraction （SPE） with Supelco C18 （500 mg/6 ml） cartridges and analyzed with HPLC on a Phenomenex C18 （250 mm × 4.6 mm, 5 μm） column with 0.1 mol/ L KH2PO4 （pH 7.0）-methanol （90：10, V/V） as mobile phase （0.5 ml/min） in isocratic mode. The identification of compounds was achieved by comparing their UV spectra with those of standard spectra in database and according to their respective time. The detection limit ranges from 0.05 to 0.2 mg/L and the accuracy of the method is between 94.71% and 97.03%.