酪蛋白水解肽对家兔细胞因子的影响

Effects of Enzymatic Hydrolysates form Casein by Pepsin on Rabbit Cytokines

为了探索酪蛋白水解肽对家兔血清中IL-1β、IL-6、IL-12、IFN-γ、TNF-α、IL-1ra、IL-4、IL-10、TGF-β细胞因子的影响,采用胰蛋白酶水解得到酪蛋白水解物溶液,经超滤后得酪蛋白水解肽。将所得水解多肽以3种方式(口服、腹腔注射、耳静脉注射)对家兔给药,分别在给药3、2、2h后,用ELISA试剂盒测定血清中IL-1β、IL-6、IL-12、IFN-γ、TNF-α、IL-1ra、IL-4、IL-10、TGF-β细胞因子的浓度。结果表明,各种作用方式IL-4、IL-6、IL-10、TNF-α、TGF-β在家兔体内的含量都明显上升,但腹腔注射酪蛋白水解多肽较其他方式作用明显,而IL-1β、IL-1ra、IL-12、IFN-γ的含量降低,最明显的作用方式为腹腔注射,说明酪蛋白水解肽通过肠黏膜系统对细胞因子网络起着重要的调节作用。从给药途径看,腹腔注射的作用效果最为显著,其次是静脉注射,口服灌胃虽达到显著水平,但变化相对较小。说明酪蛋白水解肽主要是通过肠黏膜发挥作用,可通过制备肠溶性保健食品调节人体亚健康,同时为营养组学得研究奠定基础。

Objective： To explore the effects of enzymatic hydrolysates from casein by pepsin on IL-1β, IL-6, IL-12, IFN- γ, TNF-α、 L-lra, IL-4, IL-10 and TGF- β of rabbit serum. Methods： The hydrolysates were administered to female white rabbits by gavage, intraperitoneal injection and ear intravenous injection. At 3 h, 2 h and 2 h after administration respectively, ELISA kit was used assay IL-16, IL-6, IL-12, IFN-γ, TNF-α, IL-lra, IL-4, IL-10 and TGF-β concentrations in rabbit serum. Results： The concentrations of IL-1β, IL-lra, IL- 12 and IFN-γ are reduced by the three administration methods, and the concentrations of IL-4, IL-6, IL- 10, TNF-α and TGF-β are increased. Compared with the other two administrations, intraperitoneal injection has more significant effects, which shows that cytokine network for mucosal immune system plays an important regulatory role. From the point of drug deliveries, the effect of intraperitoneal injection is the most markable, followed by ear intravenous injection, and gavage administration has no obvious effect. Conclusion： As a whole, It was suggested that the hydrolysates play an important regulatory role for body immune function by regulating cytokines level. Among the three administration methods, the most obvious method is intraperitoneal injection. The hydrolysates are managed directly by intestinal mucosal. Intraperitoneal injection is possibly a kind of the most effective drug delivery.