富铬复合营养酸奶对糖尿病模型大鼠胰岛结构及功能的保护作用

Protection of Chromium-enriched Compound Acidophilous Milk to Structure and Function of Pancreatic Islet in Alloxen-induced Diabetic Rats

目的:探讨富铬复合营养酸奶对糖尿病大鼠胰岛结构及功能的保护作用。方法:对成年Wister大鼠尾静脉注射四氧嘧啶50mg/kg,制作糖尿病模型,取模型成功的糖尿病大鼠20只随机分为糖尿病对照组和治疗组,每组10只,另取10只正常大鼠为正常对照组。正常对照组灌胃蒸馏水,糖尿病对照组灌胃普通酸奶,治疗组动物灌胃富铬复合营养酸奶。实验测定指标包括血清胰岛素、C-肽和血浆胰高血糖素含量测定及动物胰岛病理结构观察。结果:治疗组大鼠血清胰岛素、C-肽含量极显著高于糖尿病对照组(p<0.01),血浆胰高血糖素含量显著低于糖尿病对照组(p<0.05),病理切片观察和免疫组化结果显示四氧嘧啶可破坏大鼠胰岛结构和β细胞,糖尿病对照组大鼠受损伤程度尤为明显。结论富铬复合营养酸奶对四氧嘧啶致糖尿病大鼠胰岛结构及功能具有明显的保护作用。

Objective： To study the protection of chromium-enriched compound acidophilous milk to structure and function of pancreatic islet in alloxen-induced diabetic rats. Methods： Adult Wister rats were given a single shot of alloxan 50 mg/kg. After the diabetic model had been induced by aUoxen, 20 model rats were randomly divided into diabetes （DM） group and therapy group. In addition, 10 normal rats were set up as control group which were treated with intragastric administration of distilled water. While ordinary acidophilous milk and chromium-enriched compound acidophilous milk were administrated for DM group and therapy group respectively. The levels of serum insulin, C-peptide, plasm glucagons and the pathological structure of pancreatic islet were examined. Results Compared with DM group, the concentrations of serum insulin and C-peptid are significantly increased （p 〈 0.01） and the level of plasm glucagons is markedly decreased （p〈0.05） in therapy group. Moreover, the results of pathological section and immunohistochemistry demonstrated that the structure and β-cell of pancreatic islet can be damaged by alloxan, which is more obvious in DM group. Conclusion： The impaired structure and function of pancreatic islet in diabetic rats induced by alloxen can be protected noticeably by chromium-enriched compound acidophilous milk.