摘要
应用高效液相色谱法（HPLC）定性和定量检测加标香肠和水样品中相思子毒素-a（abrin-a）。在固定相为Shodex Protein KW-802．5色谱柱，流动相为0．5mol／L的磷酸盐缓冲液（PB，pH7．3），流速为1mL／min，柱温为25℃，检测波长为280llin等条件下，测定结果：abrin-a保留时间（9．80±O．02）min（n=25）；浓度与峰面积呈良好的线性关系，线性范围11．765—941．200μg/ml；最低检测限44ng；加标香肠和水样品中abrin-a回收率分别为104．6％和97．9％。测定结果表明，该方法简便、快速、灵敏度较高、重现性好。
A new high-performance liquid chromatography （HPLC） method emplyed to examine abrin-a from artificially contaminated sausage and water samples was described. Stationary phase applied was Shodex Protein KW-802.5 column, Abrin-a was separated from dopants with the mobile phase 0.5 mol/L phosphate buffered solution （PB, pH 7.3） with a flow rate of 1 mlJmin. The retention time of abrin-a was （9.80 ± 0.02 ）min （n = 25 ） . The calibration graph was linear in the range of 11.765- 941.200μg/mL abrin-a. The detection limit was 44 ng. The average recoveries of abrin-a in artificially contaminated sausage and water samples with standard abrin-a were 104.6% and 97.9% respectively. We concluded that this is a simple, sensitive and accurate method.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2008年第6期842-845,共4页
Journal of Jilin Agricultural University
基金
国家自然科学基金专项基金项目(30640049)
吉林大学农学部博士科研启动基金项目(2006)
