桑黄深层发酵上清液抗氧化活性的研究

Antioxidant activity of the supernatant fermented fluid of Phellinus igniarus

通过DPPH.和羟基自由基清除体系来研究体外桑黄深层发酵上清液的抗氧化活性。通过深层发酵得到桑黄发酵液,经过离心和旋转蒸发得到发酵上清液,用乙酸乙酯和石油醚对其进行萃取,得到不同的萃取物和萃余物,研究其抗氧化活性。结果表明,桑黄发酵上清液乙酸乙酯萃余物自由基清除能力最强,且自由基清除率随浓度增加而增强,各组分的抗氧化能力递减顺序为:乙酸乙酯萃余物>乙酸乙酯萃取物>发酵上清液>石油醚萃余物。定性实验初步验证上清液萃余物中抗氧化成分可能为黄酮、酚类、蒽醌类等。

The antioxidant activity of Phellinus igniarus submerged fermentation broth was investigated. The Phellinus igniarus submerged fermentation broth was centrifuged at high speed. After that, supernatant fluid was low press condensed and extracted by petroleum ether and ethyl acetate in detail. Five extract fractions were tested for antioxidant activity against 1,1 - Diphenyl-2- picrylhydrazyl （ DPPH · ） free radical and hydroxyl radical. According to the qualitative test, the active component was definited. In the DPPH · assay, extract of ethyl acetate showed the marked antioxidant activity and its antioxidant activity enhanced with the adding of the concentration. Antioxidant activity of other extract fractions ： ethyl acetate non-soluble substance 〉 ethyl acetate ether extract 〉 fermentation broth 〉 petroleum non-soluble substance. Results of preliminary chemical color reaction indicated that the possible effective components were flavones, phenolic anthraquinones and so on. All the results above indicated that Phellinus igniarus had marked antioxidant activity. It＇ s a good natural antioxidant and a source of antioxidant material.