摘要
建立了一种测定成分比较复杂的微生物发酵液中生物素含量的方法,确定了最佳的色谱测定条件:色谱柱为ZORBAX SB-C18(5μm,4.6×250mm),流动相为乙腈和水(体积比为1∶6,用H3PO4调节pH为3.5),柱温为30℃,流速为0.8mL/min,紫外检测波长210nm。方法的相对标准偏差在2.0%以内,回收率在90%以上,检测限为2mg/L。实验表明,此法简便、准确、快速,仅需对样品进行简单的离心、过滤即可直接进样测定。
A method for the determination of biotin in microbial fermented broth was established. The optimum chromatographic conditions were identified on ZORBAX SB- C18 column （5μm,4.6 × 250mm ）, with acetonitrile and water as mobile phase（ 1：6 by volume, pH 3.5）, column temperature of 30℃, flow rate of 0.8mL/min, and detected wavelength of 210nm. The RSD was less than 2.0% and the recovery was above 90%. The detection limit was 2mg/L The method had been successfully applied to determination of biotin in microbial fermented broth after the sample was centrifugated and filtrated,which proved to be simple,accurate and rapid.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第12期236-237,240,共3页
Science and Technology of Food Industry
