期刊文献+

利用毛细管电泳法测定乳品中乳铁蛋白方法的研究 被引量:5

Study on detection method of lactoferrin in milk product by capillary electrophoresis
下载PDF
导出
摘要 将样品经样品缓冲液处理,毛细管柱选择为直径50μm、有效长度400mm的涂层中性毛细管柱;柱温为40℃;缓冲溶液为柠檬酸缓冲液(pH为3.0±0.2);工作电压为21kV;检测波长为214nm;进样时间为5s,进行检测。结果乳铁蛋白平均回收率为75%~91%;相对标准偏差(RSD)为1.1%~2.8%;最低检出限为0.003mg/mL。该方法简便,准确,灵敏度高,是快速检测乳品中乳铁蛋白含量的最适合的分析方法。 The samples were handled by the sample buffer, using a coating neutral capillary that the diameter was 50μm and the effective length was 400mm, capillary temperature was 40℃, buffer solution was citric acide buffer (pH 3.0 ± 0.2), separated voltage was 2lkV, detection wavelength was 214nm, injection time was 5s. As the results,the average recoveries ranged from 75 %-91 % and the relative standard deviation (RSD) was 1.1 %-2.8% for lactoferrin. The limit of detection was 0.003mg/mh The present method was simple,accurate and sensitive. It was suitable for fast screening and a quantitative tool for monitoring of the lactoferrin in dairy products.
出处 《食品工业科技》 CAS CSCD 北大核心 2008年第12期241-242,247,共3页 Science and Technology of Food Industry
关键词 毛细管电泳法 乳铁蛋白 乳制品 capillary etectrophoresis(CE) tlactoferrin(LF) dairy product
  • 相关文献

参考文献7

  • 1Mafia G A, Magda M, Fabiana S. Bovine lactoferrin prevents the entry and intercelluLar spread of herpessimplex virus type 1 in Green Monkey Kidney cells [ J ]. Antiviral Res,2007 ,76 :252-262.
  • 2Giovanna L, Valeria P, Monica M, et al. Lactoferrin inhibits early steps of human BK polyomavirus infectionl [ J ]. Antiviral Res, 2006,72 : 145-152.
  • 3Strom M B, Hang B E, Rekd M Q,et al. Important structural features of 15 -- residue laetoferricin derivatives and methods for improvements of antimicrobial activity [ J ] . Biochem, 2002,80 : 65-74.
  • 4Vogel H J, Sehlbli D J, Jing W, et al. Towards a structure function analysis of bovine lactoferricin and related tryptophan- and argininecontaining peptides[ J]. Biochem,2002,80:49-63.
  • 5Jesson H, Andersen J H, Uhlin- Hanson L, et al. Anti- HSV activity of lactoferricin analogues is only partly related to their affinity for heparan suLfate [ J ]. Antiviral Research, 2004 ( 61 ) : 101-109.
  • 6M A Manso, M Miguel. Application of capillary zone electrophoresis to the characterization of the human milk protein profile and its evolution throughout lactation [ J ]. Journal of Chromatography A ,2007,1146 : 110-117.
  • 7Agostina P, Maria G A, Antonella T, et al. Bovine lactoferrin peptidic fragments involved in inhibition of Echovirus 6 in vitro infection [ J ]. Antiviral Res, 2006,69 : 98-106.

同被引文献36

  • 1许宁.高效毛细管电泳法测定牛乳铁蛋白的含量[J].中国医院药学杂志,2005,25(4):296-297. 被引量:9
  • 2曲练达,朴范泽,侯喜林,王术德.乳铁蛋白的国内外研究进展[J].黑龙江八一农垦大学学报,2005,17(6):50-54. 被引量:14
  • 3姚笠.乳铁蛋白──一种多功能免疫调节蛋白[J].国外医学(免疫学分册),1996,19(6):317-318. 被引量:9
  • 4李珊珊 王加启 魏宏阳 等.乳及乳制品中乳铁蛋自定量测定法SDS-PAGE法的建立.中国乳业,2008,(9):42-46.
  • 5Peter Riechel, Torsten Weiss, Martina Weiss, et al, Determination of the minor whey protein bovine lactoferrin in cheese whey con- centrates with capillary electrophoresis[J]. Journal of Chromatogra- phy A, 1998, 817(1-2):187-193.
  • 6KP Palmano, DF Elgar. Detection and quantitation of lactoferrin in bovine whey samples by reversed-phase high-performance liq- uid chromatography on polystyrene-divinylbenzene[J]. Journal of Chromatography A, 2002, 947(2): 307-311.
  • 7Rejman, WL Hurley, JM Bahr, Enzyme-linked immunosorbent assays of bovine lactoferrin and a 39-Kilodahon protein found in mammary secretions during involution[J]. Journal of Dairy Sci- ence, 1989, 72(2):555-560.
  • 8S Namba, T Ishii, Y Okuda, et al.Development of latex agglutina- tion turbidimetric immunoassay for the quantitative reagents of human and bovine lactoferrin[J]. Foods & Food Ingre- dients Journal of Japan,2006, 211(5): 387-397.
  • 9T Soejima, K Yamauchi, T Yamamoto, et al. Determination of bo- vine lactoferrin in lactoferrin-supplemented dairy products and raw milk by an automated latex assay[J]. Journal of Dairy Re- search, 2007, 74(1):100-105.
  • 10HE lndyk, EL Filonzi. Determination of lactoferrin in bovine milk, eolostrum and infant formulas by optical biosensor analysis [J]. International Dairy Journal, 2005, 15(5): 429-438.

引证文献5

二级引证文献23

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部