乙肝病毒突变核心蛋白基因的克隆及序列分析

Cloning and analysis of the mutational core gene of the hepatitis B virus

目的克隆发生长片段缺失的乙肝病毒核心蛋白基因（HBV-C），并对其进行DNA序列和蛋白质结构分析。方法通过PCR从1株乙型肝炎病毒中扩增得到发生长片段缺失的HBV-C基因，利用TA克隆将PCR产物克隆人pUCm—T载体并进行测序、同源性比较和蛋白质结构分析。结果PCR扩增出的HBV-C基因经序列分析表明长度为454bp，其核苷酸序列缺失了220—317bp之间的98个碱基，造成从74个氨基酸起发生移码突变。结论成功克隆发生长片段缺失的HBV-C基因，为表达及功能研究奠定了基础。

Objective To clone and analyze the deleted form of the hepatitis B virus core gene. Methods The deleted form of hepatitis B virus core gene was obtained by PCR from a hepatitis B virus genome. The PCR product was ligated into pUCm-T vector and sequenced. Result The cloned segment was 454 bp with 98 bp between 220 bp and 317 bp being lost. Conclusion The deleted form of hepatitis B virus core gene was cloned successfully which could be used for expression and function researches.