Influence of Moxibustion Serum on the Expression of Fas bcl-2 mRNA and Protein of EL-4 Lymphoma Cells

Objective： To observe the influence of moxibustion serum of mice on the expression of Fas, bcl-2 mRNA and protein of EL-4 lymphoma cells in vitro. Method： The EL-4 lymphoma cells were cultivated for 24 h in the moxibustion serum of mice. The expression of Fas and bcl-2 mRNA of EL-4 lymphoma cells were detected by in-situ hybridization method, and the protein expression of Fas and bcl-2 were observed by the immuocytochemistry method. Results： The expression of bcl-2 mRNA and protein decreased , and the expression of Fas mRNA and protein increased significantly in EL-4 cells, which were cultivated in the moxibustion serum compared those cultivated in normal mice serum （P〈0.05）. Conclusion： Moxibustion serum could down-regulate the bcl-2 mRNA and protein and up-regulate the Fas mRNA and protein of EL-4 cells.

目的:观察小鼠艾灸血清体外对 EL-4淋巴瘤细胞 Fas、bcl-2mRNA 及蛋白表达的影响。方法:采用小鼠艾灸血清培养 EL-4淋巴瘤细胞24h 后,应用原位杂交方法检测 EL-4细胞 Fas、bcl-2mRNA 的表达,免疫细胞化学方法观察 Fas、bcl-2蛋白表达。结果:与正常小鼠血清比较,艾灸血清培养 EL-4细胞后,在 bcl-2 mRNA 及蛋白水平上,阳性细胞百分率均明显降低(P<0.05),在 Fas mRNA 及蛋白水平上,阳性细胞百分率均明显升高(P<0.05)。结论:艾灸血清能够下调 EL-4细胞 bcl-2 mRNA 及蛋白的表达,上调 Fas mRNA及蛋白的表达。