摘要
采用二次回归通用旋转组合设计方法，对蛹虫草菌丝体液体发酵培养基的最佳组合进行了研究，建立了培养基的反应模型，并用实验数据验证了数学模型，对培养基最佳优化组合的各种因子、影响产量的反应规律及交互作用进行了探讨，并在此基础上进行了蛹虫草菌丝体液体发酵条件的研究。结果表明，蛹虫草菌丝体液体发酵的最适条件为：接种量６％（ｖ／ｖ），发酵初始ｐＨ７．０，培养基装量１００ｍｌ／５００ｍｌ三角瓶，发酵温度２４℃～２８℃，发酵周期９６ｈ。
he optimization of fermentation medium for mycelia production of C.militaris was studied by using a current,rotational and combinative design of quadratic regression in this experiment.The mathematical model of reaction on the media was built up.The model was of high prediction and verified by the experimental data to be reliable.The reaction regularity,relation between the two factors in the best combination of medium,the output of mycelia and mutual effect of different factors were discussed by use of the mathematical model.The optimum culture conditions were as follows:the initial pH 7.0,96 h of the culture time,6% of inoculated volume,culture temperature of 24℃～28℃ and 100ml/500ml flask of culture volume.
出处
《核农学报》
CAS
CSCD
1998年第1期35-40,共6页
Journal of Nuclear Agricultural Sciences
关键词
蛹虫草菌
菌丝体发酵生产
培养基优化
数学模型
Cordyceps militaris,mycelia fermentation production,optimization of medium,mathematical model
