体外培养时间、卵丘细胞数目对卵母细胞膜电位的影响

Influence of Time of Culture in vilre,Numbers of Cumulus Cells on Membrane Potential of Pig Oocytes

应用细胞内微电极技术，研究体外培养时间、卵丘细胞数目对猪卵母细胞模电位的影响。结果表明：体外培养时间对卵母细胞膜电位有显著影响。体外培养０ｈ膜电位为－８．９７±０．５０ｍＶ，１８ｈ膜电位为－４６．４０±１．７０ｍＶ，２４ｈ膜电位为＋１０．６０±１．００ｍＶ，３６ｈ膜电位为＋２６．５０±１．００ｍＶ，５０ｈ膜电位为＋２６．４０±１．１０ｍＶ。卵丘细胞数目对卵母细胞膜电位亦有影响。多丘卵的膜电位为－８．９７±０．５０ｍＶ，少丘卵膜电位为－１．５５±０．６５０ｍＶ，无丘卵的膜电位为＋２．４０±０．３０ｍＶ。

The effects of time of culture in vitro,numbers of cumulus cells on membrane potential of pig oocytes were studied by the introcellular microeleclrode recording method.The effect of culture in vilro on membrane potential of pig oocytes was significant.Membrane potential of oocytes at 0,18,24,36 and 50h of culture in vitrowas -8.97±0.50 mV,-46.40±1.70 mV,+10.60±1.00 mV,+26.50±1.00 mV and +26.40±1.10 mV, respectively.And the effect of numbers of cumulus cells on membrane potential of pig oocytes was also significant. The membrane potential of the oocytes enclosed with more than five layers of cumulus cells was -8.97±0.50 mV ofthe oocyles enclosed with few cumulus cells was -1.55±0.60 mV and of the oocytes with no cumulus cells was +2.40±0.30 mV.