以树突状细胞为基础的前列腺癌免疫治疗的研究

The Experimental Study About the Immunizing Treatment of Prostate Cancer Induced By Sensitized DC

探讨以人外周血单个核细胞(peripheral blood monouclear cells,PBMC)来源的树突状细胞(dendritic cell,DC)为基础的抗前列腺癌免疫作用。分离外周血PBMC,用重组人粒细胞巨噬细胞集落刺激因子(rhGM-CSF)和重组人白介素-4(rhIL-4)诱导PBMC产生DC。提取前列腺癌PC-3细胞总RNA,致敏DC并用流式细胞仪检测细胞的表面标志物CD14、CD40、CD80、CD86。致敏后的DC与淋巴细胞共同培养诱导产生特异性细胞毒性T淋巴细胞(cytotoxicTlym-phocyte,CTL),用MTT细胞毒检测试剂盒检测特异性CTL对肿瘤细胞的杀伤率。结果总RNA致敏后的DC细胞CD40、CD80、CD86显著增高,CD14显著降低(P<0.05)。前列腺癌组杀伤率(55.27+9.76)与空白对照组杀伤率(36.75+7.06)之间的差异有统计学意义(P=0.009<0.05)。胃癌对照组杀伤率(36.75+7.06)与空白对照组杀伤率(35.66+7.85)之间差异无统计学意义(P=0.834>0.05)。结论是经RNA致敏的DC可以诱导产生对前列腺癌细胞有特异性杀伤活性的CTL。

To investigate the anti-tumor effects of cytotoxic T lymphocyte（CTL） induced by sD.C. DC was induced from peripheral blood monouclear cells（PBMC） by combined rhIL-4 and rhGM-CSF. Abstract mRNA from prostate cancer cells,and sensitize DC with it. DC sensitized by mRNA（sDC）would be detected by a flow cytometer which could monitor its surface marker CD14,CD40,CD80 and CD86. sDC and lymphocyte were co-cultured to induce CTL. The anti-tumor effects of CTL was measured by using MTT kit. CD40 ,CD80 and CD86 increased significantly and CD14 decreased significantly（P%0.05）. There was significant difference between kill-rates of the prostate cancer groups and that of blank groups（P= 0. 009〈0. 05）, and no significant difference between stomach cancer groups and blank groups（ P= 0. 843〉 0.05）. Therefore,sDC could induce CTL which had specific action killing cancer cells.