高浓度葡萄糖对昆明小鼠早期胚胎发育的影响

The Effect of Hyperglycemia on Early Mouse Embryo Development

建立昆明小鼠受孕模型,分离并体外培养胚胎细胞.检测了各培养浓度下的细胞增殖、分化与凋亡.胚胎细胞在0.2mmol/L和5.56mmol/L葡萄糖浓度的KSOM培养基中能正常发育和孵化;而在浓度为15.56mmol/L和25.56mmol/L葡萄糖培养基中胚胎发育和孵化均受到损害(P<0.005),且总细胞数和内细胞团细胞数也明显减少(P<0.01),但其细胞凋亡率与0.2mmol/L和5.56mmol/L葡萄糖浓度下胚胎细胞凋亡率无显著性差异(P>0.05).随着葡萄糖浓度的增高,胚泡总的表面积无明显变化,但胚胎细胞密度呈增加趋势.高血糖对早期胚胎的发育具有毒性作用,提示高糖可能导致妊娠合并糖尿病患者的流产和胎儿畸形率升高.

Female Kuming mice were superovulated and mated in pairs. The mouse embryos were harvested and cultured in vitro in KSOM medium supplemented with 0.2, 5.56, 15.56 or 25.56 mmol/L glucose. Cell pro liferation, differentiation and apoptosis were assessed. Expected levels of blastocyst formation and hatching were seen at 0.2 and 5.56 mmol/L concentrations of glucose but both were impaired at 15.56 mmol/L and 25.56 mmol/L concentrations of glucose（P〈 0.005）. Total cell numbers and cell allocation to the inner cell mass were reduced （P〈 0.01）, but the ratio of cell apoptosis was not significantly changed in the hyperglycemic cultures （P〉0.05）. There was no significant effect on the total surface area of blastocysts, but there was a significant effect on cell density with density decreasing as glucose concentration increases（P 〈0.01）. Hyperglycemic is toxic to early embryo development. These findings hint that hyperglycemia may cause higher rates of congenital abnormality and early pregnancy loss in patients with diabetes.