高效液相色谱法测定小鼠血浆中芦荟大黄素的浓度

Determination of aloe emodin in mouse plasma by HPLC

目的:建立以高效液相色谱法测定小鼠血浆中芦荟大黄素浓度的分析方法。方法:取0.3ml小鼠血浆,二氯甲烷萃取;HPLC采用Agilent 1100高效液相色谱仪系统,以Symmetry Shield RPC18为色谱柱,流动相为甲醇-水-醋酸(65∶35∶0.2),并用荧光法测定(λex=410nm和λem=510nm)。结果:芦荟大黄素的保留时间为11.7min;芦荟大黄素浓度在10～1000ng/ml(r=0.999)的范围内具有良好的线性关系,最低检测限(LOD)和最低定量限(LOQ)分别为4.5ng/ml和5ng/ml。结论:本方法简便,准确,灵敏,特异性强,重现性好,可用于血浆中芦荟大黄素的测定及药动学研究。

Objective： To develop a HPLC method for the determination of aloe emodin （AE） in mouse plasma. Methods： 0.3 ml of plasma was taken and extracted by dichloromethane. The Agilent 1100 HPLC system was used with the Symmetry Shield RP C18, a mobile phase was methanol-water-acetic acid （65：35：0.2） and fluorescence detection was set at λex=410 nm and λem=510 nm. Results： The retention time of AE was 11.7 min, There was a good linear relationship at the range of 10 -1 000 ng/ml for aloe emodin （r=0.999）, the LOD and LOQ were 4.5 ng/ml and 5 ng/ml, respectively. Conclusion： The method is simple, accurate, sensitive, with high specificity and good reproducibility, it can be used for the determination of aloe emodin and pharmacokinetics study of aloe emodin in mice.