摘要
采用响应面法对毛云芝菌固体发酵产漆酶的培养基进行优化。首先运用部分因子筛选实验(FFD)筛选出对发酵培养基影响较大的重要因素,分别为桑叶与麸皮比例、草酸铵、葡萄糖;然后用最陡爬坡实验逼近最佳响应面区域,最后通过Box-Behnken实验设计,利用SAS进行回归分析和岭脊分析,得到主要因素的最佳比例及浓度。在优化培养基的条件下,固体发酵培养酶活较初始培养基提高了10倍。
Response surface methodology(RSM) was employed to optimize culture medium for the laccase production in solid cultivation by Coriolus hirsutus. A fractional factorial design (FFD) was used to screen out the important components for the fermentation substrate. It is inverse proportion of mulberry leaves and bran, oxalic acid ammonium and glucose. The path of steepest ascent was used to approach the optimal response re- gion. The proportion and concentrations of the significant factors were optimized consequently through Box -Behnken experiment design and using regress and ridge analysis. As the result, the production of laccase under solid culture with optimal medium was 10 times higher than under the original medium used previously.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2008年第6期1111-1116,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
广东省科技攻关项目(2006B13001004)
教育部留学回国科研启动基金项目(教外司留[2007]1108号)
关键词
毛云芝菌
漆酶
部分因子筛选
响应面法
岭脊分析
Coriolus hirsutus
laccase
fractional factorial design
response surface method
ridge analysis
optimization