丹参酮ⅡA对肥厚心肌信号转导系统蛋白激酶B通路的影响

Protective Effect of Tanshinone ⅡA on Myocardial Hypertrophy Signal Transduction System Protein Kinase B in Rats

目的:探讨丹参酮ⅡA对压力超负荷性心肌肥厚大鼠的细胞信号转导系统中蛋白激酶B(Akt)的影响。方法:健康雄性成年大鼠48只,采用胸主动脉部分缩窄术复制心肌肥厚模型大鼠40只,随机分为模型组(M组),缬沙坦组(X组)各8只,丹参酮组(D组)24只,另8只设为假手术组(S组)。术后2周,X组大鼠给予缬沙坦10mg/kg灌胃;D组大鼠按丹参酮5、10和20mg/kg剂量各8只腹腔注射;S及M组大鼠给予同等容量无菌注射用水腹腔注射。各组治疗均每日1次。8周后检测各组大鼠左室心肌质量指数(LVMI),左心室后壁(LVPW)及室间隔(IVS)厚度,比较心肌纤维横径(MFD),心肌组织中p-Akt,p-Gsk3β信号转导蛋白的量。结果:与S组比较,M组、X组和D组的大鼠左室心肌质量指数,左心室及室间隔厚度及心肌纤维横径均增加,p-Akt及p-Gsk3β升高(均P<0.05),与M组比较,X组和D组各项检测指标均下降;X组与D组间比较差异无显著性意义;D组中各剂量丹参酮治疗的大鼠间比较差异无显著性意义。结论:丹参酮ⅡA可以通过作用于蛋白激酶B(Akt)信号通路,防止心肌肥厚。

Objective： To explore the effects of tanshinone ⅡA on cell signal transduction system protein kinase B in rats with pressure overload myocardial hypertrophy. Methods： To copy the model of rats myocardial hypertrophy through adopting partial coarctation in the thoracic aorta. 48 rats were randomly divided into sham group（group S）,model group（group M）, valsartan treatment group （group X）,low-dose tanshinone treatment group （group LD）, medium-dose tanshinone treatment group （group MD）, high-dose tanshinone treatment group （groupHD）,and detected respectively left ventricular mass index（LVMI）, left ventricular posterior wall and septal thickness（LVPW, IVS） by highfrequenty uhrasonography, myocardial cells diameter by HE staining,content of p-Akt, p-Gsk3β in myocardial by western blot. Results： Compared with the sham group, left ventricular mass index, left ventricular posterior wall and septal thickness were increased（P〈0.05 ）, and myocardial cells diameter were higher,and p-Akt , p-Gsk3β were increased, in model group and all treatment group, compared with model group, left ventricular mass index, left ventricular posterior wall and septal thickness were decreased（P〈0.05）, and myocardial cells diameter were smaller, and p-Akt, p-Gsk3β were decreased in all treatment group. But there were no significant difference among the low, medium, high dose tanshinone treatment group ,and there were no significant difference among the valsartan treatment group and all dose tanshinone treatment group. Conclusion： Tanshinone UA can act on the protein kinase B （Akt） signaling pathway ,to prevent myocardial hypertrophy.