自由溶液毛细管电泳结合内标法分离测定甘草中的甘草次酸和甘草酸(英文)

Separation and determination of glycyrrhetinic acid and glycyrrhizic acid in Glycyrrhiza uralensis Fisch root by CZE with internal standard method

以双氯芬酸钠为内标,建立了一种快速、灵敏、准确的分离和测定甘草中甘草次酸和甘草酸的自由溶液毛细管电泳新方法.背景电解质是10 mmol/L硼砂(pH 8.8),分离在5 min内完成.实验证明,所建立的内标法能提高自由溶液毛细管电泳的精密度和定量的准确性.该方法有良好的线性,甘草次酸和甘草酸线性方程的相关系数分别为0.999 6和0.9991.甘草次酸和甘草酸的回收率范围分别为95.6%～100.9%和96.8%～102.9%.该方法被成功地运用于测定甘草根的最佳浸泡和煎煮时间.

A fast, sensitive and precise analytical method based on CZE with diclofenac sodium as internal standard （IS） was developed for determining glycyrrhetinic acid （GTA） and glycyrrhizic acid （GA） in Glycyrrhiza uralensis Fisch root. This analysis was carried out with 10 mmol/L borate （adjusted to pH 8.8） as a background electrolyte. The separation was achieved in 5 min. The proposed CZE method with IS has also be proven to be a feasible and attractive way for improving accuracy of the quantitation. The method resulted in excellent linearity, with correlation coefficient of regression equation of 0.999 6 and 0.999 1 for GTA and GA, respectively. Recoveries were in the range of 95.6-100.9% and 96.8%-102.9% for GTA and GA, respectively. This method has also been successfully applied to determine the optimum time of the steeping and decocting process of Glycyrrhiza uralensis Fisch root.