加味温胆汤对抑郁模型大鼠学习记忆能力及海马cAMP-PKA途径的影响

Effects of Jiawei Wendan decoction on learning and memory and cAMP-PKA pathway of hippocampal signal transduction in rats model of depression

目的:探讨加味温胆汤对抑郁模型大鼠学习记忆能力及海马信号转导cAMP-PKA途径的影响。方法:32只SD大鼠随机分为正常组、模型组、盐酸氟西汀组及加味温胆汤组,每组8只,应用孤养加慢性轻度不可预见性应激方法造模,采用Morris水迷宫实验测试大鼠空间学习记忆能力、放射免疫法测定海马cAMP含量,以及免疫组化SABC法测定海马区PKA蛋白表达水平。结果:模型组大鼠学习记忆能力显著下降(P<0.01),海马cAMP含量增加(P<0.05)、PKA表达显著减少(P<0.01)。中、西药组与模型组比较,学习记忆能力增强(P<0.05),cAMP含量下降(P<0.05),PKA蛋白表达显著上升(P<0.05)。结论:加味温胆汤可增强抑郁症大鼠学习与记忆能力,其影响机制可能与海马信号传导cAMP-PKA途径有关。

Objective：To observe effects of Jiawei Wendan decoction on learning and memory and cAMP-PKA pathway of hippocampal signal transduction in depressive rat models. Method： Depressive rat model was established by separation and chronic unpredictable mild stress. Thirty-two SD rats were selected and randomly divided into normal group, model group, Prozac group and Jiawei Wendan decoction group with 8 rats in each group. All rats were tested for spatial learning and memory in Morris water maze. Contents of cAMP were detected by radio- immunity assay,and the expression of PKA in CA3 region of the hippocampus was assayed by using immunohistochemieal method. Result： Scores of spatial learning and memory lowered remarkably in model group, contents of cAMP increased and expressions of PKA remarkably reduced in hippocampus of model rats （P〈0.01）. Compared with model group, scores of spatial learning and memory increased obviously（P〈0.05）, contents of cAMP reduced （P〈0.05） and expressions of PKA increased in drugs groups （P〈0.05）.Conclusion： These results indicate that Jiawei Wendan decoction can enhance the hippocampus-dependent spatial learning and memory function, and cAMP-PKA oathwav of hiooocamoal signal transduction mav oartieipate in the mechanism of enhanced uerformance.