软骨藻酸对原代培养大鼠神经胶质细胞膜的影响

Effects of domoic acid on membrane function of primary cultured rat glial cells

目的研究软骨藻酸（domoic acid，DA）对原代培养大鼠神经胶质细胞膜的损伤作用。方法6．4×10^-2、6．4×10^-3,6．4×10^-4μmol／L的DA作用于原代培养的大鼠神经胶质细胞24h后，分别测定细胞Na^＋-K^＋-ATPase和Ca^2＋-Mg^2＋-ATPase的活力、细胞膜流动性和通透性的变化。结果神经胶质细胞经DA处理后，Na^＋-K^＋-ATPase和Ca^2＋-Mg^2＋-ATPase的活力均明显受到抑制，细胞膜的流动性降低、通透性升高，低、中、高剂量DA染毒组荧光偏振度分别为0．0626±0．0051、0．0685±0．0097、0．0648±0．0086，微黏度分别为0．3154±0．0298、0．3510±0．0571、0．3286±0．0504，与对照组（荧光偏振度为0．0481±0．0069，微黏度为0．2338±0．0372）相比，差异均有统计学意义（P〈0．01）。结论DA能明显损害神经胶质细胞膜的功能，进而可能引发细胞的其他损伤。

Objective To study the effects of domoic acid （DA） on membrane function of primary cultured rat glial cell. Methods After the glial cells were treated with 6.4×10^-2、6.4×10^-3 and 6.4×10^-4μmol/L DA for 24 h, the activities of Na^±-K^±-ATPase and Ca^2±-Mg^2±-ATPase, the membrane fluidity and the permeability were measured to reflect the membrane function. Results After treatment of DA for 24 h, the activities of Na^±-K^±-ATPase and Ca^2±-Mg^2±-ATPase were inhibited significantly, the membrane fluidity decreased and the membrane permeability increased. The fluorescence polarization and microviscosity in the low, middle and high dosage treatment groups were 0.0626±0.0051,0.0685±0.0097,0.0648±0.0086 and 0.3154±0.0298,0.3510± 0.0571,0.3286±0.0504 respectively, compared with the control group （0.0481 ±0.0069 and 0.2338±0.0372） （P〈0.01）. Conclusion DA has obvious effects on membrane function of rat glial cells and may cause further injury on the cells.