两种小鼠卵母细胞的核成熟检验方法比较

Comparison of Two Methods for the Nucleus Maturity Test in Mouse Oocytes

将生发泡(GV)期小鼠卵母细胞置于M2培养液中培养,分别在培养0h、4h、8h和12h收集卵母细胞,采用SYTO染色和免疫荧光标记方法进行小鼠卵母细胞的核成熟检验,并将结果进行比较。可以发现:SYTO染色和免疫荧光标记方法都可用于小鼠卵母细胞的核成熟检验,二者的结果没有显著性差异,但是,SYTO染色法更为快速、简便、廉价,可用于活体染色,适合于大规模检测,可应用于哺乳动物卵母细胞的核成熟检验。

To select a simple, fast and sensitive method for the nucleus maturity detection in mammalian oocytes, GV-stage oocytes were cultured in M2 culture medium, at 0 h, 4 h, 8 h and 12 h of culture, oocytes were collected and used for nucleus maturity test by SYTO staining and immunofluorescence technique respectively. The results were compared. We found that both SYTO dyeing and immunofluorescence technique can be used for the nucleus maturity detection in mouse oocytes, and there is none distinct difference, but SYTO dyeing method is faster, cheaper and more simple for nucleus maturity detection, and it also suits to vital staining and large-scale examination, thus SYTO dyeing can be used for the nucleus maturity detection in mammalian oocytes.